Ladyzhenskaia E P, Sukhova L S, Korableva N P, Metlitskiĭ L V
Biokhimiia. 1979 Oct;44(10):1747-53.
The uptake of [14C] leucine and its incorporation into proteins of dormant and growing potato tuber buds were studied. It was found that the label uptake was increased at the beginning of the growth period, whereas the dynamics of this process were not changed in comparison with the dormant buds samples. The rate of [14C] leucine incorporation into proteins was increased in the growing buds; this increase was not, however, due to the increase in the uptake of the labelled precursor and was probably caused by activation of the protein synthesis. In contrast, the activation of protein synthesis was accompanied by changses is the dynamic incorporation of [14C] leucine into the protein at the end of dormancy. The effect of abscisic acid (10(-7) M) on the protein synthesis was not connected with its action of the uptake of labelled precursor and depended on the physiological state of buds and incubation time. A possible mechanism of regulatory effect of abscisic acid on protein synthesis in potato tuber buds is discussed.
研究了[14C]亮氨酸的摄取及其掺入休眠和生长中的马铃薯块茎芽蛋白质中的情况。结果发现,在生长初期标记物摄取增加,然而与休眠芽样品相比,该过程的动力学没有变化。[14C]亮氨酸掺入蛋白质的速率在生长芽中增加;然而,这种增加并非由于标记前体摄取的增加,可能是由蛋白质合成的激活引起的。相反,在休眠结束时,蛋白质合成的激活伴随着[14C]亮氨酸动态掺入蛋白质的变化。脱落酸(10(-7)M)对蛋白质合成的影响与其对标记前体摄取的作用无关,并且取决于芽的生理状态和孵育时间。讨论了脱落酸对马铃薯块茎芽蛋白质合成调节作用的可能机制。