Korableva N P, Ladyzhenskaia E P, Metlitskiĭ L V
Biokhimiia. 1976;41(7):1181-8.
The rate of RNA and protein synthesis in resting and growing meristem tissues from isolated tuber growth points under their incubation with labelled precursors is studied. It is found that the label incorporation into RNA is low during deep resting, protein synthesis being rather intensive. Both RNA and protein synthesis is intensified with the beginning of growth period. More homogenous in their metabolic rate set of RNAs is found in resting tissues in contrast with growing ones. The data on fractionation of total RNA preparation on MAK column and in sucrose density gradient showed that synthesis of high molecular weight RNA with metabolic period of 4 hours is stimulated in growing meristems. It is possible that synthesis of certain fractions of RNA and protein is one of regulation mechanisms for resting in meristem tissues.
研究了分离的块茎生长点在与放射性前体一起孵育时,静止和生长的分生组织中RNA和蛋白质的合成速率。结果发现,在深度静止期间,RNA的标记掺入率较低,而蛋白质合成相当活跃。随着生长阶段的开始,RNA和蛋白质的合成均增强。与生长组织相比,静止组织中RNA的代谢速率更均匀。在MAK柱上和蔗糖密度梯度中对总RNA制剂进行分级分离的数据表明,生长的分生组织中代谢周期为4小时的高分子量RNA的合成受到刺激。RNA和蛋白质某些部分的合成可能是分生组织组织静止的调节机制之一。