人类线粒体tRNALeu（CUN）中的T茎滑动决定其氨基酸负载能力。
A T-stem slip in human mitochondrial tRNALeu(CUN) governs its charging capacity.
作者信息
Hao Rui, Zhao Ming-Wei, Hao Zhan-Xi, Yao Yong-Neng, Wang En-Duo
机构信息
State Key Laboratory of Molecular Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences 320 Yue Yang Road, Shanghai 200031, People's Republic of China.
出版信息
Nucleic Acids Res. 2005 Jun 22;33(11):3606-13. doi: 10.1093/nar/gki677. Print 2005.
The human mitochondrial tRNALeu(CUN) [hmtRNALeu(CUN)] corresponds to the most abundant codon for leucine in human mitochondrial protein genes. Here, in vitro studies reveal that the U48C substitution in hmtRNALeu(CUN), which corresponds to the pathological T12311C gene mutation, improved the aminoacylation efficiency of hmtRNALeu(CUN). Enzymatic probing suggested a more flexible secondary structure in the wild-type hmtRNALeu(CUN) transcript compared with the U48C mutant. Structural analysis revealed that the flexibility of hmtRNALeu(CUN) facilitates a T-stem slip resulting in two potential tertiary structures. Several rationally designed tRNALeu(CUN) mutants were generated to examine the structural and functional consequences of the T-stem slip. Examination of these hmtRNALeu(CUN) mutants indicated that the T-stem slip governs tRNA accepting activity. These results suggest a novel, self-regulation mechanism of tRNA structure and function.
人类线粒体亮氨酰 - tRNA（CUN）[hmtRNALeu（CUN）]对应于人类线粒体蛋白质基因中亮氨酸最丰富的密码子。在此，体外研究表明，hmtRNALeu（CUN）中的U48C取代（对应于病理性T12311C基因突变）提高了hmtRNALeu（CUN）的氨酰化效率。酶促探测表明，与U48C突变体相比，野生型hmtRNALeu（CUN）转录本的二级结构更灵活。结构分析表明，hmtRNALeu（CUN）的灵活性促进了T茎滑动，从而产生两种潜在 的三级结构。生成了几个合理设计的tRNALeu（CUN）突变体，以研究T茎滑动的结构和功能后果。对这些hmtRNALeu（CUN）突变体的研究表明，T茎滑动控制着tRNA的接受活性。这些结果提示了一种tRNA结构和功能的新型自我调节机制。
Zotero 插件