Taşli Hüseyin, Bahar I Hakki
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
Jpn J Infect Dis. 2005 Jun;58(3):162-7.
TEM- and SHV-derived extended-spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae have been reported from throughout the world, but there has been limited data for the molecular characterization of these enzymes in Turkey. The aim of this study was to investigate and to type the TEM- and SHV-derived ESBLs in 63 ESBL-producing clinical isolates of Enterobacteriaceae, and it included further analysis; transfer experiments, isoelectric focusing, PCR, PCR-restriction fragment length polymorphism, and DNA sequencing. According to PCR results the transconjugant strains included 52.7% TEM, 74.3% SHV, and 32.4% of both the TEM and SHV genes. Using PCR/NheI restriction analysis, 45 of the 46 ESBL detected in transconjugants were determined to be SHV-derived. DNA sequencing was performed for the identification of TEM- and SHV-derived ESBLs for 18 selected transconjugants. SHV-2, SHV-5, and SHV-12 were detected in five, seven, and five samples, respectively. This is the first description of SHV-12 in Turkey.
世界各地均有产超广谱β-内酰胺酶（ESBLs）的肠杆菌科细菌的报道，这些酶由TEM型和SHV型衍生而来，但土耳其关于这些酶分子特征的数据有限。本研究旨在调查并分型63株产ESBLs的临床肠杆菌科分离株中TEM型和SHV型衍生的ESBLs，并进行进一步分析；包括接合转移实验、等电聚焦、聚合酶链反应（PCR）、PCR-限制性片段长度多态性分析及DNA测序。根据PCR结果，接合转移菌株中包含52.7%的TEM基因、74.3%的SHV基因以及32.4%同时含有TEM和SHV基因的菌株。利用PCR/NheI限制性分析，在接合转移菌株中检测到的46种ESBLs中有45种被确定为SHV型衍生。对18株选定的接合转移菌株进行DNA测序以鉴定TEM型和SHV型衍生的ESBLs。分别在5份、7份和5份样本中检测到SHV-2、SHV-5和SHV-12。这是土耳其首次报道SHV-12。
