Ben Yebdri Fethia, Aazaz Abderrahmane, Ye Kai, Ma Hui-Wen, Tong Li-Heng
College of Pharmacy, Wuhan University, Wuhan 430072, China.
Sheng Wu Gong Cheng Xue Bao. 2004 Sep;20(5):683-8.
In order to study the feasibility of E2 gene fragment of hepatitis virus G(HGV) as a component of DNA vaccine against the hepatitis virus G infection, a 559bp DNA fragment encoding HGV E2 was cloned into plasmid pCMV-S from pThioHis-E2 in the same reading frame with HBsAg gene to form a recombinant plasmid named pCMV-S-E2. BALB/c mice of Kunming strain were immunized with purified plasmid DNA of pCMV-S-E2 by intra-muscularly inoculation. The immunizations were boosted twice at an interval of 14 days. The whole blood was collected from mice orbit on the day-8 after the last boost. Mice sera were screened by ELISA to determine the humoral immune response using E2-GST fusion protein as the immobilized antigen and the sera from mice immunized with pCMV-S as control. The result indicated that the immunization with plasmid DNA of pCMV-S-E2 could induce quite strong humoral immune response.
为研究庚型肝炎病毒（HGV）E2基因片段作为抗庚型肝炎病毒感染DNA疫苗组分的可行性，将编码HGV E2的559bp DNA片段从pThioHis-E2克隆至质粒pCMV-S中，使其与乙肝表面抗原（HBsAg）基因处于同一阅读框，构建成重组质粒pCMV-S-E2。采用昆明系BALB/c小鼠，通过肌肉注射纯化的pCMV-S-E2质粒DNA进行免疫。免疫接种间隔14天加强免疫2次。末次加强免疫后第8天，从小鼠眼眶采集全血。以E2-GST融合蛋白为固定抗原，通过ELISA筛选小鼠血清，以确定体液免疫应答，并用pCMV-S免疫小鼠的血清作为对照。结果表明，pCMV-S-E2质粒DNA免疫可诱导较强的体液免疫应答。
