Zhang Peixun, He Xiangjun, Liu Kaiyan, Zhao Fuqiang, Fu Zhongguo, Zhang Dianying, Zhang Qi, Jiang Baoguo
People Hospital, Peking University Medical College, Beijing, China.
Artif Cells Blood Substit Immobil Biotechnol. 2004;32(4):509-18. doi: 10.1081/bio-200039608.
Bone marrow stromal cells (MSCs) have been shown to differentiate into various lineage cells including neural cells in vitro and in vivo. We therefore examined whether MSCs can differentiate into Schwann cells in injured peripheral nerves, After cultured in vitro, PKH-67-labeled MSCs were injected into the mechanically injured rat sciatic nerves. Three weeks after injection, immunofluorescent examinations were carried out. MSCs had been incorporated around the injured nerves and differentiated into Schwann cells. MSCs had accumulated mainly in the epineurium around the injured nerve. The incorporated cells partially expressed GFAP, S-100, and P75. These results confirmed the possibility that MSCs have the ability to differentiate into Schwann cells, and that injection of MSCs into the injured peripheral nerve would help repair damaged nerve.
骨髓基质细胞（MSCs）已被证明在体外和体内均可分化为包括神经细胞在内的各种谱系细胞。因此，我们研究了MSCs是否能在受损的周围神经中分化为雪旺细胞。体外培养后，将PKH-67标记的MSCs注入机械损伤的大鼠坐骨神经。注射三周后，进行免疫荧光检查。MSCs已整合到受损神经周围并分化为雪旺细胞。MSCs主要聚集在受损神经周围的神经外膜中。整合的细胞部分表达GFAP、S-100和P75。这些结果证实了MSCs具有分化为雪旺细胞的能力，并且将MSCs注入受损的周围神经有助于修复受损神经。
