Department of Anatomy, Guangdong Medical College, Zhangjiang, People's Republic of China.
J Tissue Eng Regen Med. 2012 Jul;6(7):579-88. doi: 10.1002/term.467. Epub 2011 Sep 20.
To better understand the use of Schwann cells (SCs) and bone marrow stromal cells (BMSCs) together for nerve repair, we studied whether interactions between these two cell types by diffusible molecules can enhance their utility. In the present study, a co-culture system was established to allow BMSCs and SCs grow in the same culture medium but without physical contact. Before co-culture, the adult SCs were expanded until confluent. The adult BMSCs were cultured until P10 with CD29 and CD44 positive but CD45 negative. After 4 days in culture, > 80% of the BMSCs in the co-culture system showed both GFAP- and S-100-positive, but < 6% of the BMSCs in control culture system showed both GFAP- and S-100-positive. Meanwhile, 68.76% of the SCs in co-culture system showed S-100-positive, which was > 42.03% of the SCs in control culture system. Furthermore, the in vivo study also confirmed that differentiated BMSCs exert a more beneficial effect on repairing injured sciatic nerve function and axonal regeneration than undifferentiated BMSCs. These results indicate that the two most widely used cell types for promoting peripheral nerve regeneration may interact synergistically to aid their roles in peripheral nerve repair.
为了更好地理解施万细胞（SCs）和骨髓基质细胞（BMSCs）在神经修复中的联合应用，我们研究了这两种细胞类型之间通过扩散分子相互作用是否可以增强它们的效用。在本研究中，建立了共培养系统，使 BMSCs 和 SCs 在相同的培养基中生长，但没有物理接触。在共培养之前，将成年 SCs 扩增至汇合。将成年 BMSCs 培养至 P10，CD29 和 CD44 阳性，但 CD45 阴性。培养 4 天后，共培养系统中 >80%的 BMSCs 显示出 GFAP 和 S-100 阳性，但对照培养系统中仅有 <6%的 BMSCs 显示出 GFAP 和 S-100 阳性。同时，共培养系统中 68.76%的 SCs 显示出 S-100 阳性，而对照培养系统中仅有 42.03%的 SCs 显示出 S-100 阳性。此外，体内研究也证实，分化的 BMSCs 在修复损伤的坐骨神经功能和轴突再生方面比未分化的 BMSCs 发挥更有益的作用。这些结果表明，两种最常用于促进周围神经再生的细胞类型可能会协同作用，以增强它们在外周神经修复中的作用。
