在非洲爪蟾早期发育过程中，xBtg - x调节Wnt/β - 连环蛋白信号通路。
xBtg-x regulates Wnt/beta-Catenin signaling during early Xenopus development.
作者信息
Wessely Oliver, Kim James I, Tran Uyen, Fuentealba Luis, De Robertis E M
机构信息
Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.
出版信息
Dev Biol. 2005 Jul 1;283(1):17-28. doi: 10.1016/j.ydbio.2005.03.033.
In Xenopus, two signaling systems, maternal beta-Catenin and Nodal-related, are required for induction of the Spemann organizer and establishment of the body plan. By screening cDNA macroarrays for genes activated by these two signaling pathways, we identified Xenopus xBtg-x, a novel member of the Btg/Tob gene family of antiproliferative proteins. We show that xBtg-x is expressed in the dorsal mesendoderm (Spemann organizer tissue) of gastrula stage embryos and that its expression is regulated by both beta-Catenin and Nodal-related signals. Microinjection of synthetic xBtg-x mRNA into Xenopus embryos induced axis duplication and completely rescued the ventralizing effects of UV irradiation through the activation of the canonical Wnt/beta-Catenin signaling pathway. Interestingly, xBtg-x stimulated beta-Catenin-dependent transcription without affecting the stability of beta-Catenin protein. These data suggest that xBtg-x is a novel component of the Wnt/beta-Catenin signaling pathway regulating early embryonic patterning.
在非洲爪蟾中，母源β-连环蛋白和Nodal相关这两个信号系统对于诱导施佩曼组织者以及建立身体模式是必需的。通过筛选cDNA宏阵列以寻找由这两个信号通路激活的基因，我们鉴定出了非洲爪蟾xBtg-x，它是抗增殖蛋白Btg/Tob基因家族的一个新成员。我们发现xBtg-x在原肠胚期胚胎的背侧中内胚层（施佩曼组织者组织）中表达，并且其表达受β-连环蛋白和Nodal相关信号两者的调控。将合成的xBtg-x mRNA显微注射到非洲爪蟾胚胎中会诱导轴重复，并通过激活经典的Wnt/β-连环蛋白信号通路完全挽救紫外线照射的腹化效应。有趣的是，xBtg-x刺激β-连环蛋白依赖性转录，而不影响β-连环蛋白蛋白的稳定性。这些数据表明xBtg-x是调控早期胚胎模式形成的Wnt/β-连环蛋白信号通路的一个新组分。
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