Faure Patrice, Ducros Véronique, Couzy François, Favier Alain, Ferry Monique
Laboratoire HP2, Faculté de Médecine et de Pharmacie, BP 217, Grenoble, France.
Nutrition. 2005 Jul-Aug;21(7-8):831-7. doi: 10.1016/j.nut.2004.12.008.
We evaluated the effect of age and institutionalization on zinc metabolism by using a stable isotope technique.
This was a randomized case-control study. Three groups were recruited: nine young women (group 1, ages 36+/-1 y) as controls, nine free-living elderly women (group 2, ages 72+/-2 y), and nine institutionalized women (group 3, ages 73+/-2 y). Only women were recruited to obtain homogeneous groups. The study was set in a Valence hospital (France) in the geriatric department (headed by Dr. Ferry). The experimental design of the study was reviewed and approved by the local ethical committee, and all participants signed a consent form. No subject dropped out of the study. A zinc stable isotope label (0.73 mg of (70)Zn) was injected intravenously into patients and measured by inductively coupled plasma mass spectrometry.
Decay curves of the plasma (70)Zn followed a one-compartment kinetic leading to the determination of one pool. The size of this pool suggested that it corresponded to the liver compartment. The size of this pool was significantly smaller in elderly people. (70)Zn plasma resident time was significantly longer in elderly individuals, and shorter in institutionalized than in free-living elderly subjects.
These data suggest that the zinc metabolism of elderly women is related to lifestyle or its consequences and to age. Moreover, we have demonstrated that kinetic studies using stable isotopes of zinc can provide novel information on exchangeable zinc pools in clinical situations.
我们运用稳定同位素技术评估年龄和机构化生活对锌代谢的影响。
这是一项随机病例对照研究。招募了三组人群：九名年轻女性（第1组，年龄36±1岁）作为对照组，九名居家养老的老年女性（第2组，年龄72±2岁），以及九名机构化生活的女性（第3组，年龄73±2岁）。仅招募女性以确保研究组的同质性。该研究在法国瓦朗斯市一家医院的老年病科（由费里医生负责）开展。本研究的实验设计经当地伦理委员会审查并批准，所有参与者均签署了知情同意书。无受试者退出研究。将锌稳定同位素标记物（0.73毫克的(70)Zn）静脉注射给患者，并通过电感耦合等离子体质谱法进行测量。
血浆(70)Zn的衰变曲线遵循单室动力学，由此确定了一个池。该池的大小表明其对应于肝脏部分。老年人这个池的大小明显较小。(70)Zn在老年人血浆中的驻留时间显著更长，且在机构化生活的老年人中比在居家养老的老年人中更短。
这些数据表明老年女性的锌代谢与生活方式或其后果以及年龄有关。此外，我们已证明使用锌稳定同位素进行的动力学研究能够在临床情况下提供关于可交换锌池的新信息。
