茶多酚表没食子儿茶素没食子酸酯（EGCG）和表没食子儿茶素（EGC）可抑制癌细胞中人端粒酶逆转录酶（hTERT）的mRNA表达。
The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells.
作者信息
Lin Shu-Chun, Li Wan-Chun, Shih Jing-Wen, Hong Kuo-Fu, Pan Yen-Ru, Lin Jing-Jer
机构信息
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Li-Nong St, Sec., 2, No155, Peitou, Taipei 112, Taiwan, ROC.
出版信息
Cancer Lett. 2006 May 8;236(1):80-8. doi: 10.1016/j.canlet.2005.05.003. Epub 2005 Jun 21.
Tea polyphenols have inhibitive effects for carcinogenesis. A reporter system controlled by hTERT promoter was constructed to evaluate the effects of tea polyphenols, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC) on the repression of hTERT transcription. The hTERT promoter activity was selectively repressed by 20-40 microM EGCG and EGC in a dose- and time-dependent manner. Real-time RT-PCR confirmed that the endogenous hTERT mRNA level was decreased in H1299, OECM-1 and SAS cells treated with EGCG or EGC. Our results identified the repression activities of EGCG and EGC toward telomerase expression that might be linked to inhibition of carcinoma cell growth. This cell-based reporter system is useful for screening drugs targeting hTERT repression.
茶多酚对致癌作用具有抑制效果。构建了一个由人端粒酶逆转录酶（hTERT）启动子控制的报告系统，以评估茶多酚、（-）-表没食子儿茶素-3-没食子酸酯（EGCG）和（-）-表没食子儿茶素（EGC）对hTERT转录抑制的作用。20 - 40微摩尔的EGCG和EGC以剂量和时间依赖性方式选择性地抑制hTERT启动子活性。实时逆转录聚合酶链反应（Real-time RT-PCR）证实，用EGCG或EGC处理的H1299、OECM-1和SAS细胞中内源性hTERT mRNA水平降低。我们的结果确定了EGCG和EGC对端粒酶表达的抑制活性，这可能与癌细胞生长的抑制有关。这种基于细胞的报告系统可用于筛选靶向hTERT抑制的药物。
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