Hansen A P, Heinis J J
Department of Food Science, North Carolina State University, Raleigh 27695-7624.
J Dairy Sci. 1992 May;75(5):1211-5. doi: 10.3168/jds.S0022-0302(92)77869-2.
The effect of sodium caseinate and whey protein concentrate on benzaldehyde, d-limonene, and citral flavor intensity was determined by quantitative descriptive analysis deviation from reference using a 12-member trained panel. The concentrations for the benzaldehyde, d-limonene, and citral flavor intensity references were 17.8, 53.0, and 19.8 ppm, respectively. The concentration for both protein references was .25%. Flavored protein solutions were held for 17 h at 6 degrees C and contained benzaldehyde (17.8 ppm), d-limonene (53 ppm), or citral (19.8 ppm) and 2.5% sucrose with 0, .125, .25, or .5% protein. Benzaldehyde flavor intensity declined as the whey protein concentrate concentration increased from 0 to .5%. There was no significant difference in benzaldehyde flavor intensity with casein compared with the reference. The d-limonene flavor intensity declined as the protein concentration increased. Panelists found no significant drop in citral flavor intensity with casein or whey protein. Decreased benzaldehyde and d-limonene flavor intensity in the presence of whey protein concentrate or casein may be due to nonpolar interactions (casein), interaction with nonpolar binding sites, cysteine-aldehyde condensation, or Schiff base formation (whey protein concentrate).
使用一个由12名经过培训的小组成员,通过与参考标准的定量描述分析偏差,测定了酪蛋白酸钠和乳清蛋白浓缩物对苯甲醛、d-柠檬烯和柠檬醛风味强度的影响。苯甲醛、d-柠檬烯和柠檬醛风味强度参考标准的浓度分别为17.8、53.0和19.8 ppm。两种蛋白质参考标准的浓度均为0.25%。调味蛋白质溶液在6℃下保存17小时,含有苯甲醛(17.8 ppm)、d-柠檬烯(53 ppm)或柠檬醛(19.8 ppm)以及2.5%的蔗糖,蛋白质含量分别为0、0.125%、0.25%或0.5%。随着乳清蛋白浓缩物浓度从0增加到0.5%,苯甲醛风味强度下降。与参考标准相比,酪蛋白的苯甲醛风味强度没有显著差异。随着蛋白质浓度增加,d-柠檬烯风味强度下降。小组成员发现,酪蛋白或乳清蛋白存在时,柠檬醛风味强度没有显著下降。乳清蛋白浓缩物或酪蛋白存在时,苯甲醛和d-柠檬烯风味强度降低可能是由于非极性相互作用(酪蛋白)、与非极性结合位点的相互作用、半胱氨酸-醛缩合或席夫碱形成(乳清蛋白浓缩物)。