Emmett Stevan R, Greenfield Susan A
University Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK.
J Chem Neuroanat. 2005 Jul;30(1):34-44. doi: 10.1016/j.jchemneu.2005.04.004.
The aim of this study was to investigate the relationship between the cells possessing the alpha3 or alpha5 nicotinic acetylcholine receptor subunits and the enzyme acetylcholinesterase, with respect to tyrosine hydroxylase immunoreactive dopaminergic neurons in the rat substantia nigra. Most, but certainly not all, acetylcholinesterase immunoreactive cells were located in the pars compacta. In the substantia nigra pars compacta there were in turn two populations of acetylcholinesterase containing neurons: those that were tyrosine hydroxylase reactive and those that were not. Double label studies, that included an antibody immunoreactive against a common immunogen on alpha1 of muscle and alpha3 and alpha5 neuronal nicotinic acetylcholine receptor subunits, revealed that nearly all nicotinic receptor positive cells were also tyrosine hydroxylase neurons. However, a minority non-tyrosine hydroxylase population was alpha3- and/or alpha5-nAChR positive and these were always AChE-immunoreactive. In summary, there appears to be a close correlation between nicotinic receptors and acetylcholinesterase in the substantia nigra, irrespective of the transmitter phenotype in different neuronal subpopulations.
本研究旨在探讨在大鼠黑质中，拥有α3或α5烟碱型乙酰胆碱受体亚基的细胞与乙酰胆碱酯酶之间，相对于酪氨酸羟化酶免疫反应性多巴胺能神经元的关系。大多数（但肯定不是全部）乙酰胆碱酯酶免疫反应性细胞位于致密部。在黑质致密部，又存在两类含有乙酰胆碱酯酶的神经元：酪氨酸羟化酶反应性神经元和非反应性神经元。双重标记研究，包括一种针对肌肉α1以及α3和α5神经元烟碱型乙酰胆碱受体亚基上共同免疫原的抗体免疫反应，显示几乎所有烟碱型受体阳性细胞也是酪氨酸羟化酶神经元。然而，少数非酪氨酸羟化酶群体是α3和/或α5 - nAChR阳性，且这些细胞总是乙酰胆碱酯酶免疫反应性的。总之，在黑质中，无论不同神经元亚群中的递质表型如何，烟碱型受体与乙酰胆碱酯酶之间似乎都存在密切关联。
