Gore Christopher J, Hopkins Will G, Burge Caroline M
Department of Physiology, Australian Institute of Sport, P.O. Box 176, Belconnen, Australian Capital Territory, Australia.
J Appl Physiol (1985). 2005 Nov;99(5):1745-58. doi: 10.1152/japplphysiol.00505.2005. Epub 2005 Jun 23.
The volume of red blood cells (V(RBC)) is used routinely in the diagnostic workup of polycythemia, in assessing the efficacy of erythropoietin administration, and to study factors affecting oxygen transport. However, errors of various methods of measurement of V(RBC) and related parameters are not well characterized. We meta-analyzed 346 estimates of error of measurement of V(RBC) for techniques based on Evans blue (V(RBC,Evans)), 51chromium-labeled red blood cells (V(RBC,51Cr)), and carbon monoxide (CO) rebreathing (V(RBC,CO)), as well as hemoglobin mass with the carbon-monoxide method (M(Hb,CO)), in athletes and active and inactive subjects undergoing various experimental and control treatments lasting minutes to months. Subject characteristics and experimental treatments had little effect on error of measurement, but measures with the smallest error showed some increase in error with increasing time between trials. Adjusted to 1 day between trials and expressed as coefficients of variation, mean errors for M(Hb,CO) (2.2%; 90% confidence interval 1.4-3.5%) and V(RBC,51Cr) (2.8%; 2.4-3.2%) were much less than those for V(RBC,Evans) (6.7%; 4.9-9.4%) and V(RBC,CO) (6.7%; 3.4-14%). Most of the error of V(RBC,Evans) was due to error in measurement of volume of plasma via Evans blue dye (6.0%; 4.5-7.8%), which is the basis of V(RBC,Evans). Most of the error in V(RBC,CO) was due to estimates from laboratories with a relatively large error in M(Hb,CO), the basis of V(RBC,CO). V(RBC,51Cr) and M(Hb,CO) are the best measures for research on blood-related changes in oxygen transport. With care, V(RBC,Evans) is suitable for clinical applications of blood-volume measurement.
红细胞体积（V(RBC)）常用于真性红细胞增多症的诊断检查、评估促红细胞生成素的给药效果以及研究影响氧运输的因素。然而，V(RBC)及相关参数的各种测量方法的误差尚未得到充分表征。我们对基于伊文思蓝法（V(RBC,Evans)）、51铬标记红细胞法（V(RBC,51Cr)）和一氧化碳（CO）重呼吸法（V(RBC,CO)）测量V(RBC)的误差估计值进行了荟萃分析，同时还分析了采用一氧化碳法测量血红蛋白质量（M(Hb,CO)）的误差估计值，涉及运动员以及接受从数分钟到数月不等的各种实验和对照治疗的活跃和不活跃受试者。受试者特征和实验治疗对测量误差影响不大，但误差最小的测量方法显示，随着两次试验之间时间间隔的增加，误差会有所增加。调整为两次试验间隔1天并以变异系数表示，M(Hb,CO)的平均误差（2.2%；90%置信区间1.4 - 3.5%）和V(RBC,51Cr)的平均误差（2.8%；2.4 - 3.2%）远小于V(RBC,Evans)的平均误差（6.7%；4.9 - 9.4%）和V(RBC,CO)的平均误差（6.7%；3.4 - 14%）。V(RBC,Evans)的大部分误差是由于通过伊文思蓝染料测量血浆体积时的误差（6.0%；4.5 - 7.8%），而这是V(RBC,Evans)的基础。V(RBC,CO)的大部分误差是由于来自M(Hb,CO)误差相对较大的实验室的估计值，而M(Hb,CO)是V(RBC,CO)的基础。V(RBC,51Cr)和M(Hb,CO)是研究与血液相关的氧运输变化的最佳测量方法。谨慎使用的话，V(RBC,Evans)适用于血容量测量的临床应用。
