Khomenkov V G, Shevelev A B, Zhukov V G, Kurlovich A E, Zagustina N A, Popov V O
Prikl Biokhim Mikrobiol. 2005 May-Jun;41(3):298-302.
Genes for catechol 1,2- and 2,3-dioxygenases were cloned. These enzymes hold important positions in the ortho and meta pathways of the metabolism of aromatic carbons by microbial associations that consume the following volatile organic compounds in pilot minireactors: toluene, styrene, ethyl benzene, o-xylene, m-xylene, and naphthalene. Genes of both pathways were found in an association consuming m-xylene; only genes of the ortho pathway were found in associations consuming o-xylene, styrene, and ethyl benzene, and only genes of the meta pathway were found in associations consuming naphthalene and toluene. Genes of the ortho pathway (C120) cloned from associations consuming o-xylene and ethyl benzene were similar to corresponding genes located on the pND6 plasmid of Pseudomonas putida. Genes of the ortho pathway from associations consuming o-xylene and m-xylene were similar to chromosomal genes of P. putida. Genes of the meta pathway (C230) from associations consuming toluene and naphthalene were similar to corresponding genes formerly found in plasmids pWWO and pTOL.
儿茶酚1,2 - 双加氧酶和2,3 - 双加氧酶的基因被克隆。这些酶在微生物群落代谢芳香碳的邻位和间位途径中占据重要地位，这些微生物群落在小型试验反应器中消耗以下挥发性有机化合物：甲苯、苯乙烯、乙苯、邻二甲苯、间二甲苯和萘。在消耗间二甲苯的群落中发现了两条途径的基因；在消耗邻二甲苯、苯乙烯和乙苯的群落中仅发现了邻位途径的基因，而在消耗萘和甲苯的群落中仅发现了间位途径的基因。从消耗邻二甲苯和乙苯的群落中克隆的邻位途径基因（C120）与恶臭假单胞菌pND6质粒上的相应基因相似。从消耗邻二甲苯和间二甲苯的群落中得到的邻位途径基因与恶臭假单胞菌的染色体基因相似。从消耗甲苯和萘的群落中得到的间位途径基因（C230）与先前在质粒pWWO和pTOL中发现的相应基因相似。
