Kumar S, Laouar L, Pritchard D I, Lowe K C
Department of Life Science, University of Nottingham, University Park, United Kingdom.
J Parasitol. 1992 Jun;78(3):550-2.
A new method is described for the isolation of cultured nematode larvae. This allows effective separation of larvae from fecal contamination, exsheathed larvae from cast sheaths, and viable larvae from nonviable larvae. The method involves the use of cellulose strips and has been assessed using larvae from 2 hookworm species, Necator americanus and Ancylostoma ceylanicum. Pretreatment of the cellulose strips with 1.0% (w/v) of the nonionic surfactant, Pluronic F-68, significantly increased larval recovery of both species.
本文描述了一种分离培养线虫幼虫的新方法。该方法能够有效地将幼虫与粪便污染物分离，将脱鞘幼虫与蜕下的鞘分离，以及将活幼虫与死幼虫分离。该方法使用了纤维素条，并已用来自两种钩虫——美洲板口线虫和锡兰钩虫的幼虫进行了评估。用1.0%（w/v）的非离子表面活性剂普朗尼克F - 68对纤维素条进行预处理，显著提高了两种钩虫幼虫的回收率。
