Kulak Wojciech, Sobaniec Wojciech, Solowej Elzbieta, Sobaniec Henryka
Department of Pediatric Neurology and Rehabilitation, Medical University of Białystok, Waszyngtona 17, 15-274 Białystok, Poland.
Life Sci. 2005 Oct 28;77(24):3031-6. doi: 10.1016/j.lfs.2005.02.031. Epub 2005 Jun 23.
Impaired antioxidant mechanisms are unable to inactivate free radicals that may induce a number of pathophysiological processes and result in cell injury. Thus, any abnormality in antioxidant defense systems could affect neurodevelopmental processes and could have an important role in the etiology of cerebral palsy (CP). The plasma levels of lipid peroxidation as plasma levels of malondialdehyde (MDA), activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) in plasma and erythrocytes were investigated in 34 CP children and compared with 61 normal controls. SOD, GPx and GR activities were spectrophotometrically assayed. Activities of SOD, GPx and GR in plasma did not differ significantly between CP children and the control group. Activities of erythrocyte GR in the CP patients were significantly lower compared with controls. MDA concentration did not differ statistically between the CP children and healthy subjects. In conclusion our results suggest that increased activities of erythrocyte GPx and decreased erythrocyte GR activities might be due to lesser physical activity of children with CP.
抗氧化机制受损会导致自由基无法失活，进而引发一系列病理生理过程并造成细胞损伤。因此，抗氧化防御系统的任何异常都可能影响神经发育过程，并在脑瘫（CP）的病因中发挥重要作用。本研究检测了34例脑瘫患儿血浆中脂质过氧化水平（以丙二醛（MDA）血浆水平表示）以及血浆和红细胞中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的活性，并与61例正常对照进行比较。采用分光光度法测定SOD、GPx和GR的活性。脑瘫患儿与对照组血浆中SOD、GPx和GR的活性无显著差异。与对照组相比，脑瘫患儿红细胞GR的活性显著降低。脑瘫患儿与健康受试者的MDA浓度在统计学上无差异。总之，我们的结果表明，红细胞GPx活性增加和红细胞GR活性降低可能是由于脑瘫患儿身体活动较少所致。
