Stallmann Hein P, Faber Chris, Bronckers Antonius L J J, de Blieck-Hogervorst Jolanda M A, Brouwer Carlo P J M, Amerongen Arie V Nieuw, Wuisman Paul I J M
Orthopedic Surgery, VU Medical Center, PO box 7057, 1007 MB Amsterdam, The Netherlands.
Peptides. 2005 Dec;26(12):2355-9. doi: 10.1016/j.peptides.2005.05.014. Epub 2005 Jun 23.
In order to analyze the clinical potential of two antimicrobial peptides, human lactoferrin 1-11 (hLF1-11) and synthetic histatin analogue Dhvar-5, we measured the killing effect on bacteria, and the potential toxicity on erythrocytes and bone cells. The antimicrobial activity was determined in a killing assay on six strains, including methicillin resistant Staphylococcus Aureus. The effect on human erythrocytes and MC3T3 mouse bone cells was measured with a hemolysis assay and a viability assay, respectively. Both hLF1-11 and Dhvar-5 dose-dependently killed all bacterial strains, starting at concentrations of 6 microg/mL. hLF1-11 had no effect on mammalian cells at concentrations up to 400 microg/mL, but Dhvar-5 induced significant hemolysis (37% at 200 microg/mL) and bone cell death (70% at 400 microg/mL). This indicates that both peptides are able to kill various resistant and non-resistant bacteria, but Dhvar-5 may exert a cytotoxic effect on host cells at higher concentrations.
为了分析两种抗菌肽——人乳铁蛋白1 - 11（hLF1 - 11）和合成组蛋白类似物Dhvar - 5的临床潜力，我们测定了它们对细菌的杀伤作用以及对红细胞和骨细胞的潜在毒性。在对包括耐甲氧西林金黄色葡萄球菌在内的六种菌株进行的杀伤试验中测定了抗菌活性。分别用溶血试验和活力试验测定了它们对人红细胞和MC3T3小鼠骨细胞的影响。hLF1 - 11和Dhvar - 5均呈剂量依赖性地杀死所有细菌菌株，起始浓度为6微克/毫升。hLF1 - 11在浓度高达400微克/毫升时对哺乳动物细胞无影响，但Dhvar - 5诱导了显著的溶血（200微克/毫升时为37%）和骨细胞死亡（400微克/毫升时为70%）。这表明这两种肽都能够杀死各种耐药和非耐药细菌，但Dhvar - 5在较高浓度时可能对宿主细胞产生细胞毒性作用。
