Berger L, El-Alfy M, Martel C, Labrie F
Oncology and Molecular Endocrinology Research Center, Laval University Medical Center (CHUL), 2705 Laurier Boulevard, Quebec City, Que., Canada G1V 4G2.
J Steroid Biochem Mol Biol. 2005 Jul;96(2):201-15. doi: 10.1016/j.jsbmb.2005.02.018.
To assess the specific estrogenic and/or androgenic effects of a potential novel hormone replacement therapy, we have examined the morphology of the rat vagina 9 months after ovariectomy (OVX) and treatment of OVX animals with dehydroepiandrosterone (DHEA), conjugated estrogens Premarin and the selective estrogen receptor modulator Acolbifene. OVX led to atrophy and inflammatory changes while Acolbifene reduced the inflammation incidence and induced mucification of the vaginal epithelium. Premarin induced a typical keratinized stratified squamous epithelium while DHEA induced stimulation of the vaginal epithelium, with mucous cells typical of an androgenic effect, combined with increased collagen fiber compactness of the lamina propria. On the other hand, after OVX, the vaginal muscle layer decreased by 46%, an effect which was 41 and 100% reversed by DHEA and Premarin, respectively. The present data show particularly interesting effects of DHEA on the three layers of the vaginal wall, namely a highly mucified epithelium, an increased muscularis thickness and increased collagen fiber compactness in the lamina propria. DHEA exerts both androgenic and estrogenic effects on the vaginal mucosa, thus providing a more physiological replacement therapy.
为评估一种潜在新型激素替代疗法的特定雌激素和/或雄激素效应，我们检查了大鼠卵巢切除术后9个月的阴道形态，以及用脱氢表雄酮（DHEA）、结合雌激素普瑞马林和选择性雌激素受体调节剂阿考比芬治疗卵巢切除动物后的阴道形态。卵巢切除导致萎缩和炎症变化，而阿考比芬降低了炎症发生率并诱导阴道上皮黏液化。普瑞马林诱导典型的角化复层鳞状上皮，而DHEA诱导阴道上皮刺激，伴有雄激素效应典型的黏液细胞，同时固有层胶原纤维致密性增加。另一方面，卵巢切除术后，阴道肌层减少了46%，DHEA和普瑞马林分别使这一效应逆转了41%和100%。目前的数据显示DHEA对阴道壁三层有特别有趣的作用，即高度黏液化的上皮、肌层厚度增加和固有层胶原纤维致密性增加。DHEA对阴道黏膜发挥雄激素和雌激素效应，从而提供一种更接近生理状态的替代疗法。
