Sourla A, Flamand M, Bélanger A, Labrie F
Laboratory of Molecular Endocrinology, CHUQ Research Center and Laval University, Québec, Canada.
J Steroid Biochem Mol Biol. 1998 Aug;66(3):137-49. doi: 10.1016/s0960-0760(98)00052-1.
Dehydroepiandrosterone (DHEA) applied on the dorsal skin of ovariectomized animals, at the twice daily dose of 30 mg, resulted in a complete reversal of the vaginal atrophy seen 1, 3 and 6 months after ovariectomy, and induced proliferation and mucification of the vaginal epithelium. A similar mucification of the vaginal epithelium related to androgenic action was observed in intact rats treated with DHEA. While treatment of intact rats with DHEA resulted in a significant increase in uterine weight at 1 month, the value of the same parameter decreased by approximately 30% after 3 and 6 months of DHEA administration. In ovariectomized DHEA-treated animals, uterine weight was increased at all time intervals. At histopathological examination, following DHEA administration to intact animals, stimulation was seen in the myometrial layers of the uterus whereas atrophy, involving especially the endometrium, became apparent after 3 and 6 months of treatment. In ovariectomized animals, the endometrium remained atrophic at all time intervals during DHEA treatment and the uterine epithelium thus remained atrophic under DHEA treatment. Examination of the effect of DHEA on the vaginal epithelium indicates that local application of DHEA on the vaginal mucosa is approximately 10-fold more efficient than application at a distant site on the skin. Reversal of the ovariectomy-induced increase in serum LH levels was also observed after DHEA treatment. The present data suggest that DHEA possesses a tissue-specific action, through its local transformation into active estrogens in the vaginal epithelium while the uterine epithelium remains atrophic. In addition, the site of administration of DHEA appears to be a significant factor, at least for its stimulatory effect on the vaginal mucosa.
将脱氢表雄酮（DHEA）以每日两次、每次30毫克的剂量涂抹于去卵巢动物的背部皮肤，可使去卵巢后1个月、3个月和6个月出现的阴道萎缩完全逆转，并诱导阴道上皮细胞增殖和黏液化。在用DHEA处理的完整大鼠中也观察到了与雄激素作用相关的阴道上皮细胞类似的黏液化现象。虽然用DHEA处理完整大鼠1个月后子宫重量显著增加，但在给予DHEA 3个月和6个月后，该参数的值下降了约30%。在接受DHEA处理的去卵巢动物中，子宫重量在所有时间间隔均增加。在组织病理学检查中，给完整动物施用DHEA后，子宫肌层出现刺激现象，而在处理3个月和6个月后，萎缩现象变得明显，尤其是子宫内膜。在去卵巢动物中，在DHEA处理期间，子宫内膜在所有时间间隔均保持萎缩状态，因此子宫上皮在DHEA处理下也保持萎缩状态。对DHEA对阴道上皮作用的研究表明，将DHEA局部应用于阴道黏膜比在皮肤远处应用的效率高约10倍。DHEA处理后还观察到去卵巢诱导的血清促黄体生成素（LH）水平升高的现象得到逆转。目前的数据表明，DHEA具有组织特异性作用，它在阴道上皮中局部转化为活性雌激素，而子宫上皮则保持萎缩状态。此外，DHEA的给药部位似乎是一个重要因素，至少对于其对阴道黏膜的刺激作用而言是如此。
