Arriaga-Pizano Lourdes, Jimenez-Zamudio Luis, Vadillo-Ortega Felipe, Martinez-Flores Alfonso, Herrerias-Canedo Tomas, Hernandez-Guerrero Cesar
Department of Ultrastructure, Instituto Nacional de Perinatologia, Lomas Virreyes, Mexico City, Mexico.
J Soc Gynecol Investig. 2005 Jul;12(5):335-42. doi: 10.1016/j.jsgi.2005.02.005.
Human pregnancy disorders such as preeclampsia are thought to involve variations in cytokine levels. It has been proposed that, in preeclamptic women, a balance favoring the Th1-type over the Th2-type cytokine profile determines local or systemic immunologic responses to pregnancy and that this may cause defective placental implantation and placental ischemia, which activate systemic endothelial cells. The purpose of this study was to determine whether cytokine expression differs in the maternal, choriodecidual, and fetal compartments, and between women with or without preeclampsia.
Plasma concentrations of interferon gamma (IFNgamma), interleukin (IL)-2, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA) in samples obtained from maternal peripheral blood (MPB), choriodecidual (CD), and fetal cord (FC) blood compartments of 17 women with preeclampsia and in 15 normotensive women. Intracellular concentrations of IFNgamma and IL-2 in T lymphocytes were assessed by flow cytometry.
Plasma IFNgamma concentrations in both MPB and CD compartments were significantly higher in preeclamptic than in normotensive women. Maternal plasma IL-4 concentration was significantly lower in preeclamptic than in normotensive women. Intracellular IFNgamma and IL-2 concentrations did not differ significantly between preeclamptic and normotensive women.
The dominant Th1-type over Th2-type cytokine profile is evident in MPB, but not in the CD and FC blood compartments. This might reflect the complex cytokine networks in the fetal-placental interface and might involve trophoblasts or decidual and endothelial cells, which could account for the increased plasma IFNgamma concentration and T-helper cell number.
诸如先兆子痫等人类妊娠疾病被认为与细胞因子水平的变化有关。有人提出，在先兆子痫女性中，细胞因子谱倾向于Th1型而非Th2型的平衡决定了对妊娠的局部或全身免疫反应，并且这可能导致胎盘植入缺陷和胎盘缺血，从而激活全身内皮细胞。本研究的目的是确定细胞因子表达在母体、绒毛膜蜕膜和胎儿部分之间，以及在有或没有先兆子痫的女性之间是否存在差异。
通过酶联免疫吸附测定（ELISA）测量了17例先兆子痫女性和15例血压正常女性的母体外周血（MPB）、绒毛膜蜕膜（CD）和胎儿脐带（FC）血样中γ干扰素（IFNγ）、白细胞介素（IL）-2、IL-4和IL-10的血浆浓度。通过流式细胞术评估T淋巴细胞中IFNγ和IL-2的细胞内浓度。
先兆子痫女性的MPB和CD部分的血浆IFNγ浓度均显著高于血压正常女性。先兆子痫女性的母体血浆IL-4浓度显著低于血压正常女性。先兆子痫女性和血压正常女性之间的细胞内IFNγ和IL-2浓度没有显著差异。
Th1型细胞因子谱在MPB中占主导地位，而在CD和FC血样中则不明显。这可能反映了胎儿-胎盘界面中复杂的细胞因子网络，并且可能涉及滋养层细胞或蜕膜和内皮细胞，这可能解释了血浆IFNγ浓度和辅助性T细胞数量的增加。
