Thorpe A J, Kotz C M
Department of Neuroscience, University of Minnesota, 1334 Eckles Avenue, Saint Paul, MN 55108, USA.
Brain Res. 2005 Jul 19;1050(1-2):156-62. doi: 10.1016/j.brainres.2005.05.045.
Due to the nature of processing within the accumbens shell (AccSh) and the presence of orexin receptors and varicosities within the AccSh, we hypothesized that orexin A may partly regulate feeding behavior and locomotor activity via signaling in this site. To test this hypothesis, male Sprague-Dawley rats were implanted with guide cannulae directed to the medial portion of the AccSh. Orexin A (0, 100, 500, and 1000 pmol, in 0.5 microl artificial cerebrospinal fluid) was infused into the AccSh and feeding behavior and locomotor activity were monitored. The effect of pretreatment with an orexin 1 receptor antagonist (SB334867A) on orexin A-induced feeding and locomotor activity was assessed. Orexin A augmented feeding in the 0-1 h and 1-2 h post-infusion interval (P = 0.0058 and P = 0.025, respectively) and stimulated locomotor activity in the 30-60 min, 60-90 min, and 90-120 min post-infusion intervals (P <or= 0.0001, P = 0.0056 and P = 0.046, respectively). Orexin A-induced feeding was significantly attenuated by preadministration of SB334867A in the 0-1 h post-infusion time interval (P = 0.03). Orexin A-induced locomotor activity was not affected by SB334867A. These data support the hypothesis that the AccSh is a site of orexin A modulation of feeding behavior and locomotor activity.
由于伏隔核壳（AccSh）内的处理性质以及AccSh内存在食欲素受体和曲张体，我们推测食欲素A可能通过该部位的信号传导部分调节摄食行为和运动活动。为了验证这一假设，将雄性Sprague-Dawley大鼠植入导向AccSh内侧部分的引导套管。将食欲素A（0、100、500和1000皮摩尔，溶于0.5微升人工脑脊液中）注入AccSh，并监测摄食行为和运动活动。评估了用食欲素1受体拮抗剂（SB334867A）预处理对食欲素A诱导的摄食和运动活动的影响。食欲素A在注入后0 - 1小时和1 - 2小时的时间段内增加了摄食量（分别为P = 0.0058和P = 0.025），并在注入后30 - 60分钟、60 - 90分钟和90 - 120分钟的时间段内刺激了运动活动（分别为P≤0.0001、P = 0.0056和P = 0.046）。在注入后0 - 1小时的时间段内预先给予SB334867A可显著减弱食欲素A诱导的摄食（P = 0.03）。食欲素A诱导的运动活动不受SB334867A的影响。这些数据支持了以下假设：AccSh是食欲素A调节摄食行为和运动活动的部位。
