Grassi Manuela, Volpe Elisabetta, Colizzi Vittorio, Mariani Francesca
Institute Neurobiology and Molecular Medicine, Molec. Med. Section, National Research Council, Via del Fosso del Cavaliere, 100, 00133, Rome, Italy.
J Microbiol Methods. 2006 Mar;64(3):406-10. doi: 10.1016/j.mimet.2005.05.002. Epub 2005 Jun 24.
The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.
使用实时聚合酶链反应（PCR）检测低丰度信使核糖核酸（mRNA）和/或富含鸟嘌呤-胞嘧啶（GC）的靶标非常困难，通常需要繁琐的优化程序。这项研究表明，甲酰胺是一种有用的PCR添加剂，可提高SYBR Green实时PCR检测感染样本中低丰度分枝杆菌RNA的灵敏度和特异性。
