Pizzato Massimo, Erlwein Otto, Bonsall David, Kaye Stephen, Muir David, McClure Myra O
Jefferiss Trust Laboratories, Wright-Fleming Institute, Imperial College, London W2 1PG, United Kingdom.
J Virol Methods. 2009 Mar;156(1-2):1-7. doi: 10.1016/j.jviromet.2008.10.012. Epub 2008 Dec 5.
PCR-enhanced reverse transcriptase assays (PERT) are sensitive tools for the detection of retroviruses in biological samples. The adaptation of real-time PCR techniques based on fluorescent probes (F-PERT) has added a reliable quantitative capacity to the assay. In the interest of economy and time, the SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT). This assay can be completed in 2h, is linear over six orders of magnitude and can be used to quantify retroviruses belonging to divergent species, such as the human immunodeficiency virus type 1 (HIV-1), murine leukemia virus (MLV) and prototypic foamy virus (PFV).
聚合酶链反应增强逆转录酶检测法（PERT）是检测生物样品中逆转录病毒的灵敏工具。基于荧光探针的实时聚合酶链反应技术（F-PERT）的应用为该检测法增添了可靠的定量能力。出于经济和时间方面的考虑，采用基于SYBR Green I的实时检测系统建立了一种简便的一步法PERT检测法（SG-PERT）。该检测法可在2小时内完成，在六个数量级范围内呈线性，可用于定量多种不同种类的逆转录病毒，如1型人类免疫缺陷病毒（HIV-1）、鼠白血病病毒（MLV）和原型泡沫病毒（PFV）。
