一种用于能量过滤透射电子断层扫描的公共软件（EFTET-J）：应用于对巨型管虫体内细菌中颗粒内含物的研究

A public software for energy filtering transmission electron tomography (EFTET-J): application to the study of granular inclusions in bacteria from Riftia pachyptila.

作者信息

Boudier T, Lechaire J P, Frébourg G, Messaoudi C, Mory C, Colliex C, Gaill F, Marco S

机构信息

Institut Curie, UMR168 and LRC-CEA 34v. 11, rue Pierre et Marie Curie, 75005 Paris, France.

出版信息

J Struct Biol. 2005 Aug;151(2):151-9. doi: 10.1016/j.jsb.2005.05.004.

DOI:10.1016/j.jsb.2005.05.004

PMID:15979897

Abstract

Energy-filtering transmission electron microscopy (EFTEM) allows the determination of elemental distributions out of a sequence of energy filtered images. Combined with electron tomography, EFTEM is a powerful tool to obtain three-dimensional chemical maps from sub-cellular structures. However, there is no existing software in the public-domain for the computation and analysis of 3D-chemical maps. Here, we present a Java-based program to compute 3D-elemental distribution. This program is available as a set of plug-ins for the public-domain Java image processing program Image J inspired by NIH Image. Its implemented algorithms have been successfully applied to the three-dimensional localization of iron granules in semi thin (200 nm) epon sections from the vent worm Riftia pachyptalia.

摘要

能量过滤透射电子显微镜（EFTEM）能够从一系列能量过滤图像中确定元素分布。结合电子断层扫描技术，EFTEM是一种从亚细胞结构获取三维化学图谱的强大工具。然而，目前公共领域中没有用于三维化学图谱计算和分析的软件。在此，我们展示了一个基于Java的程序来计算三维元素分布。该程序作为一组插件提供，用于受美国国立卫生研究院图像（NIH Image）启发的公共领域Java图像处理程序Image J。其实现的算法已成功应用于来自巨型管虫（Riftia pachyptalia）半薄（200 nm）环氧树脂切片中铁颗粒的三维定位。