Aronova M A, Leapman R D
National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
MRS Bull. 2012 Jan;37(1):53-62. doi: 10.1557/mrs.2011.329.
The high sensitivity of electron energy loss spectroscopy (EELS) for detecting light elements at the nanoscale makes it a valuable technique for application to biological systems. In particular, EELS provides quantitative information about elemental distributions within subcellular compartments, specific atoms bound to individual macromolecular assemblies, and the composition of bionanoparticles. The EELS data can be acquired either in the fixed beam energy-filtered transmission electron microscope (EFTEM) or in the scanning transmission electron microscope (STEM), and recent progress in the development of both approaches has greatly expanded the range of applications for EELS analysis. Near single atom sensitivity is now achievable for certain elements bound to isolated macromolecules, and it becomes possible to obtain three-dimensional compositional distributions from sectioned cells through EFTEM tomography.
电子能量损失谱（EELS）在纳米尺度检测轻元素方面具有高灵敏度，这使其成为应用于生物系统的一项有价值的技术。特别是，EELS提供了关于亚细胞区室内元素分布、与单个大分子组装体结合的特定原子以及生物纳米颗粒组成的定量信息。EELS数据可以在固定束能量过滤透射电子显微镜（EFTEM）或扫描透射电子显微镜（STEM）中获取，并且这两种方法的最新进展极大地扩展了EELS分析的应用范围。现在，对于与分离的大分子结合的某些元素可实现近乎单原子的灵敏度，并且通过EFTEM断层扫描从切片细胞中获得三维成分分布成为可能。
