Zhang Ruifu, Cui Zhongli, Jiang Jiandong, He Jian, Gu Xiangyang, Li Shunpeng
Department of Microbiology, MOA Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, People's Republic of China.
Can J Microbiol. 2005 Apr;51(4):337-43. doi: 10.1139/w05-010.
Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced. Sequence analysis revealed that their hydrolase genes were conserved, and that the G+C content of the mpd genes were distinctly different from that of the chromosome-located 16S rRNA gene, suggesting that the mpd gene could be transferred and expressed among a variety of bacterial hosts.
从长期受甲基对硫磷污染的土壤中分离出7株甲基对硫磷降解菌，发现它们分别属于假氨基杆菌属、无色杆菌属、布鲁氏菌属和慢生根瘤菌属。使用mpd基因探针进行的Southern印迹分析表明，它们的水解酶基因与嗜水气单胞菌属菌株M6的mpd基因相似，且均位于染色体上。从这7株有机磷农药降解菌的基因组DNA构建了基因文库，并克隆和测序了它们的mpd基因。序列分析表明，它们的水解酶基因是保守的，且mpd基因的G+C含量与位于染色体上的16S rRNA基因明显不同，这表明mpd基因可以在多种细菌宿主之间转移和表达。
