College of Life Sciences, Nanjing Agricultural University, Nanjing, People's Republic of China.
Biodegradation. 2010 Sep;21(5):785-92. doi: 10.1007/s10532-010-9343-2. Epub 2010 Feb 24.
A rod-shaped, gram-negative bacterium Stenotrophomonas sp. SMSP-1 was isolated from the sludge of a wastewater treating system of a pesticide manufacturer. Strain SMSP-1 could hydrolyze methyl parathion to p-nitrophenol (PNP) and dimethyl phosphorothioate but could not degrade PNP further. Strain SMSP-1 was able to hydrolyze other organophosphate pesticides, including fenitrothion, ethyl parathion, fenthion, and phoxim, but not chlorpyrifos. A 4395-bp DNA fragment, including an organophosphorus hydrolase encoding gene ophc2, was cloned from the chromosome of strain SMSP-1 using the shotgun technique. Its sequence analysis showed that ophc2 was associated with a typical mobile element ISPpu12 consisting of tnpA (encoding a transposase), lspA (encoding a lipoprotein signal peptidase), and orf1 (encoding a CDF family heavy metal/H(+) antiporter). The ophc2 gene was effectively expressed in E. coli. This is the second report of cloning the ophc2 gene and the first report of this gene from the genus of Stenotrophomonas.
从一家农药制造商废水处理系统的污泥中分离到一株棒状、革兰氏阴性细菌 Stenotrophomonas sp. SMSP-1。SMSP-1 菌株可以将甲基对硫磷水解为对硝基苯酚（PNP）和二甲硫代磷酸酯，但不能进一步降解 PNP。SMSP-1 菌株能够水解其他有机磷农药，包括杀螟松、乙基对硫磷、倍硫磷和辛硫磷，但不能水解氯吡硫磷。使用鸟枪法从 SMSP-1 菌株的染色体中克隆出一个 4395-bp 的 DNA 片段，该片段包含一个有机磷水解酶编码基因 ophc2。序列分析表明，ophc2 与一个由 tnpA（编码转座酶）、lspA（编码脂蛋白信号肽酶）和 orf1（编码 CDF 家族重金属/H(+) 外排泵）组成的典型移动元件 ISPpu12 相关。ophc2 基因在大肠杆菌中得到有效表达。这是克隆 ophc2 基因的第二份报告，也是首次从 Stenotrophomonas 属报告该基因。
