Department of Agronomy and Plant Genetics and the Plant Molecular Genetics Institute, University of Minnesota, 55108, St. Paul, MN, USA.
Theor Appl Genet. 1989 Dec;78(6):761-7. doi: 10.1007/BF00266655.
Experiments were conducted to determine the chromosomal location of the gene conditioning overproduction of a methionine-rich, 10-K zein in maize kernels of line BSSS53. In addition, the chromosomal location of the structural gene encoding the overproduced protein was determined. Whereas the structural gene, designated Zps10/(22), was found to be located on the long arm of chromosome 9 near the centromere, the locus regulating overproduction of the zein protein was mapped to the short arm of chromosome 4. This regulatory gene has been designated Zpr10/(22). Regulation of 10-K zein production by Zpr10/(22) is, therefore, via a trans-acting mechanism.
进行了实验以确定调节富含蛋氨酸的 10-K 玉米醇溶蛋白在 BSSS53 品系玉米子粒中超量表达的基因在染色体上的位置。此外，还确定了编码过量表达蛋白的结构基因在染色体上的位置。虽然将这个结构基因命名为 Zps10/(22)，但它被发现位于 9 号染色体的长臂靠近着丝粒处，而调节玉米醇溶蛋白过量产生的基因座则被定位在 4 号染色体的短臂上。这个调节基因被命名为 Zpr10/(22)。因此，Zpr10/(22)通过反式作用机制来调节 10-K 玉米醇溶蛋白的产生。
