Ruta Veronica, Longo Chiara, Lepri Andrea, De Angelis Veronica, Occhigrossi Sara, Costantino Paolo, Vittorioso Paola
Department of Biology and Biotechnology, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
Plants (Basel). 2020 Feb 8;9(2):218. doi: 10.3390/plants9020218.
The DOF (DNA binding with one finger) family of plant-specific transcription factors (TF) was first identified in maize in 1995. Since then, DOF proteins have been shown to be present in the whole plant kingdom, including the unicellular alga . The DOF TF family is characterised by a highly conserved DNA binding domain (DOF domain), consisting of a CXC-X-CXC motif, which is able to form a zinc finger structure. Early in the study of DOF proteins, their relevance for seed biology became clear. Indeed, the PROLAMIN BINDING FACTOR (PBF), one of the first DOF proteins characterised, controls the endosperm-specific expression of the zein genes in maize. Subsequently, several DOF proteins from both monocots and dicots have been shown to be primarily involved in seed development, dormancy and germination, as well as in seedling development and other light-mediated processes. In the last two decades, the molecular network underlying these processes have been outlined, and the main molecular players and their interactions have been identified. In this review, we will focus on the DOF TFs involved in these molecular networks, and on their interaction with other proteins.
植物特异性转录因子（TF）的DOF（单指DNA结合）家族于1995年在玉米中首次被鉴定出来。从那时起，DOF蛋白已被证明存在于整个植物界，包括单细胞藻类。DOF转录因子家族的特征是具有一个高度保守的DNA结合结构域（DOF结构域），由一个能够形成锌指结构的CXC-X-CXC基序组成。在对DOF蛋白的早期研究中，它们与种子生物学的相关性就已明确。实际上，最早被鉴定的DOF蛋白之一——醇溶蛋白结合因子（PBF），控制着玉米中醇溶蛋白基因的胚乳特异性表达。随后，来自单子叶植物和双子叶植物的几种DOF蛋白已被证明主要参与种子发育、休眠和萌发，以及幼苗发育和其他光介导的过程。在过去的二十年里，这些过程背后的分子网络已被勾勒出来，并且主要的分子参与者及其相互作用也已被确定。在这篇综述中，我们将重点关注参与这些分子网络的DOF转录因子，以及它们与其他蛋白质的相互作用。
