Jiang Feng, Ye Xuecheng, Liu Xiang, Fincher Lauren, McKearin Dennis, Liu Qinghua
Department of Biochemistry, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Genes Dev. 2005 Jul 15;19(14):1674-9. doi: 10.1101/gad.1334005. Epub 2005 Jun 28.
In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.
在黑腹果蝇中,Dicer-2/R2D2和Dicer-1分别产生小干扰RNA(siRNA)和微小RNA(miRNA)。在此,我们鉴定出一种新型双链RNA结合蛋白,将其命名为R3D1-L,它在体外和体内均能与Dicer-1形成稳定的复合物。虽然通过RNA干扰耗尽R3D1-L会导致S2细胞中前体miRNA(pre-miRNA)积累,但重组R3D1-L在体外可增强Dicer-1产生miRNA的能力。此外,R3D1缺陷会导致果蝇雌雄两性的miRNA生成缺陷和严重不育。因此,R3D1-L在miRNA生物合成过程中与Dicer-1协同发挥作用,是果蝇生殖发育所必需的。