一个保守的Swi2/Snf2 ATP酶基序将ATP水解与染色质重塑偶联起来。
A conserved Swi2/Snf2 ATPase motif couples ATP hydrolysis to chromatin remodeling.
作者信息
Smith Corey L, Peterson Craig L
机构信息
Program in Molecular Medicine, University of Massachusetts Medical School, Biotech 2, Suite 210, Worcester, Massachusetts 01605, USA.
出版信息
Mol Cell Biol. 2005 Jul;25(14):5880-92. doi: 10.1128/MCB.25.14.5880-5892.2005.
Abstract
Yeast (Saccharomyces cerevisiae) SWI/SNF is a prototype for a large family of ATP-dependent chromatin-remodeling enzymes that facilitate numerous DNA-mediated processes. Swi2/Snf2 is the catalytic subunit of SWI/SNF, and it is the founding member of a novel subfamily of the SF2 superfamily of DNA helicase/ATPases. Here we present a functional analysis of the diagnostic set of helicase/ATPase sequence motifs found within all Swi2p/Snf2p family members. Whereas many of these motifs play key roles in ATP binding and/or hydrolysis, we identify residues within conserved motif V that are specifically required to couple ATP hydrolysis to chromatin-remodeling activity. Interestingly, motif V of the human Swi2p/Snf2p homolog, Brg1p, has been shown to be a possible hot spot for mutational alterations associated with cancers.
摘要
酵母（酿酒酵母）SWI/SNF是一大类ATP依赖型染色质重塑酶的原型，这些酶促进众多DNA介导的过程。Swi2/Snf2是SWI/SNF的催化亚基，它是DNA解旋酶/ATP酶的SF2超家族一个新亚家族的创始成员。在这里，我们对所有Swi2p/Snf2p家族成员中发现的解旋酶/ATP酶序列基序的诊断集进行了功能分析。虽然这些基序中的许多在ATP结合和/或水解中起关键作用，但我们确定了保守基序V内的残基，这些残基是将ATP水解与染色质重塑活性偶联所特需的。有趣的是，人类Swi2p/Snf2p同源物Brg1p的基序V已被证明是与癌症相关的突变改变的一个可能热点。
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