Lilius E M, Kankaanpää A, Lahti R
Department of Biochemistry, University of Turku, Finland.
J Biolumin Chemilumin. 1992 Apr;7(2):117-22. doi: 10.1002/bio.1170070204.
A novel quantitative nonlabel immunoassay is described. It is based on the recognition of antigen-antibody complexes by the Fc-receptors of phagocytic leukocytes and the subsequent activation of these cells. Activation which is proportional to the amount of immune complexes present can be detected by measuring the intensity of chemiluminescence emitted by the activated cells. In addition to determinations of an antigen and an antibody, the binding capacity of complement to antigen-antibody complexes can be estimated.
描述了一种新型的定量非标记免疫测定法。它基于吞噬性白细胞的Fc受体对抗抗原-抗体复合物的识别以及随后这些细胞的激活。通过测量被激活细胞发出的化学发光强度，可以检测到与存在的免疫复合物量成比例的激活。除了测定抗原和抗体外，还可以估计补体与抗原-抗体复合物的结合能力。
