[幽门螺杆菌感染与残胃黏膜中白细胞介素-8表达的关系]

[The relationship between Helicobacter pylori infection and IL-8 expression in remnant stomach mucosa].

作者信息

Xing Cheng-zhong, Guo Xiao-lin, Xu Hui-mian, Yuan Yuan

机构信息

Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Jul;21(4):503-5.

PMID:15989802

Abstract

AIM

To explore the relationship between helicobacter pylori (Hp) infection and expression of interleukin-8 (IL-8) in the remnant stomach mucosa.

METHODS

Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to detect Hp infection (by H.E. and Giemsa staining) and IL-8 expression (by real-time semi-quantitative RT-PCR).

RESULTS

Hp infection was detected in 65.5% (38/58)of the remnant stomach mucosa. In 34 patients with Billroth I (BI) anastomosis, IL-8 in corpus was significantly higher in Hp-positive patients than that in Hp-negative patients (0.11+/-0.07 vs 0.02+/-0.01, P<0.05). In 24 patients with Billroth II (BII) anastomosis, IL-8 in stoma and corpus in Hp-positive patients was significantly higher than that in Hp-negative patients (P<0.05).

CONCLUSION

Hp infection induces IL-8 expression in remnant stomach mucosa. In corpus, IL-8 mRNA expression is mainly related with Hp infection, while in stoma, IL-8 mRNA expressions may be related with bile reflux besides Hp infection.

摘要

目的

探讨幽门螺杆菌（Hp）感染与残胃黏膜白细胞介素-8（IL-8）表达之间的关系。

方法

对58例行胃切除术的患者进行上消化道内镜检查。从吻合口和胃体上部大弯处获取活检标本，检测Hp感染（采用苏木精-伊红染色和吉姆萨染色）及IL-8表达（采用实时半定量逆转录聚合酶链反应）。

结果

在65.5%（38/58）的残胃黏膜中检测到Hp感染。在34例行毕Ⅰ式（BI）吻合术的患者中，胃体部Hp阳性患者的IL-8水平显著高于Hp阴性患者（0.11±0.07对0.02±0.01，P<0.05）。在24例行毕Ⅱ式（BII）吻合术的患者中，Hp阳性患者吻合口和胃体部的IL-8水平显著高于Hp阴性患者（P<0.05）。