双链RNA对斑节对虾细胞中黄头病毒复制的沉默作用
Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA.
作者信息
Tirasophon Witoon, Roshorm Yaowaluck, Panyim Sakol
机构信息
The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakornpathom 73170, Thailand.
出版信息
Biochem Biophys Res Commun. 2005 Aug 19;334(1):102-7. doi: 10.1016/j.bbrc.2005.06.063.
RNA interference (RNAi) has been shown to inhibit viral replication in some animals and plants. Whether the RNAi is functional in shrimp remains to be demonstrated. In vitro transcribed dsRNAs of YHV helicase, polymerase, protease, gp116, and gp64 were transfected into shrimp primary cell culture and found to inhibit YHV replication. dsRNA targeted to nonstructural genes (protease, polymerase, and helicase) effectively inhibited YHV replication. Those targeted structural genes (gp116 and gp64) were the least effective. These findings are the first evidence that RNAi-mediated gene silencing is operative in shrimp cells. This could be a powerful tool for studying gene function and to develop effective control of viral infection in shrimp.
RNA干扰（RNAi）已被证明可在一些动植物中抑制病毒复制。RNAi在对虾中是否起作用仍有待证实。将体外转录的对虾黄头病毒解旋酶、聚合酶、蛋白酶、gp116和gp64的双链RNA转染到对虾原代细胞培养物中，发现其可抑制对虾黄头病毒的复制。靶向非结构基因（蛋白酶、聚合酶和解旋酶）的双链RNA可有效抑制对虾黄头病毒的复制。而靶向结构基因（gp116和gp64）的双链RNA效果最差。这些发现首次证明RNAi介导的基因沉默在对虾细胞中起作用。这可能是研究基因功能以及开发有效控制对虾病毒感染的有力工具。
Zotero 插件