Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Virus Res. 2014 Aug 30;189:133-5. doi: 10.1016/j.virusres.2014.05.022. Epub 2014 Jun 2.
The suppression of viral replication by double-stranded RNAs (dsRNA) specific to mRNAs of either virus or host genes has been widely investigated as a possible shrimp disease therapy. PmYRP65, a yellow head virus (YHV) receptor, was previously identified and characterized in the black tiger shrimp, Penaeus monodon. In our previous study, entry of YHV into cells of the Oka organ of P. monodon required the host receptor PmYRP65 and silencing of PmYRP65 in vitro led to a complete suppression of YHV replication in the cells. In this study, PmYRP65 was shown to be in vivo suppressed by dsRNA specific for PmYRP65, leading to inhibition of YHV replication and almost complete abolition of shrimp mortality following YHV challenge. Targeting PmYRP65 could be an effective YHV antiviral shrimp strategy.
双链 RNA（dsRNA）特异性抑制病毒或宿主基因 mRNA 的病毒复制已被广泛研究作为一种可能的虾病治疗方法。黄头病毒（YHV）受体 PmYRP65 先前在斑节对虾（Penaeus monodon）中被鉴定和表征。在我们之前的研究中，YHV 进入斑节对虾 Oka 器官细胞需要宿主受体 PmYRP65，体外沉默 PmYRP65 导致细胞中 YHV 复制完全被抑制。在这项研究中，PmYRP65 被特异性针对 PmYRP65 的 dsRNA 体内抑制，导致 YHV 复制抑制和 YHV 攻毒后虾死亡率几乎完全消除。针对 PmYRP65 可能是一种有效的 YHV 抗病毒虾策略。
