van Luyn M J, van Wachem P B, Nieuwenhuis P, Jonkman M F
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Biomaterials. 1992;13(5):267-75. doi: 10.1016/0142-9612(92)90049-t.
Wound dressings may induce cytotoxic effects. In this study, we check several, mostly commercially available, wound dressings for cytotoxicity. We used our previously described, newly developed and highly sensitive 7 d methylcellulose cell culture with fibroblasts as the test system. Cytotoxicity is assessed by monitoring cell growth inhibition, supported by cell morphological evaluation using light and transmission electron microscopy. We tested conventional wound dressings, polyurethane-based films, composites, hydrocolloids and a collagen-based dressing. It was shown that only 5 out of 16 wound dressings did not induce cytotoxic effects. All 5 hydrocolloids were found to inhibit cell growth (greater than 70%), while cells had strongly deviant morphologies. The remaining wound dressings showed medium cytotoxic effects, with cell growth inhibition, which varied from low (+/- 15%), medium-low (+/- 25%) to medium-high (+/- 50%). Measurable cytotoxic effects of dressings detected in vitro are likely to interfere with wound healing when applied in vivo. The results are discussed in view of the clinical uses with contaminated wounds, impaired epithelialization or hypergranulation.
伤口敷料可能会产生细胞毒性作用。在本研究中，我们检测了几种（大多为市售的）伤口敷料的细胞毒性。我们使用了之前描述的、新开发的且高度灵敏的以成纤维细胞为测试系统的7天甲基纤维素细胞培养法。通过监测细胞生长抑制来评估细胞毒性，并借助光学显微镜和透射电子显微镜进行细胞形态学评估来辅助判断。我们测试了传统伤口敷料、聚氨酯基薄膜、复合材料、水胶体和胶原基敷料。结果显示，16种伤口敷料中只有5种没有诱导细胞毒性作用。发现所有5种水胶体均抑制细胞生长（大于70%），同时细胞具有严重异常的形态。其余伤口敷料表现出中等细胞毒性作用，细胞生长抑制程度各异，从低（±15%）、中低（±25%）到中高（±50%）不等。在体外检测到的敷料可测量的细胞毒性作用在体内应用时可能会干扰伤口愈合。结合污染伤口、上皮化受损或肉芽组织增生过度的临床应用情况对结果进行了讨论。
