Viennet C, Bride J, Gabiot A C, Humbert P
Engineering and Cutaneous Biology Laboratory, School of Medicine and Pharmacy, Besançon, France.
J Wound Care. 2003 Nov;12(10):385-90. doi: 10.12968/jowc.2003.12.10.26544.
We compared the effects on cultured human fibroblasts of a new non-adhesive wound dressing, Urgotul, with five other wound dressings. Urgotul is a hydrocolloid dressing; the comparator dressings included impregnated gauze and modern wound dressings.
Cultures in monolayer were used to study the morphology and growth of fibroblasts. The Bell model of cultured dermis equivalents was used to investigate myofibroblast differentiation. These cultures were labelled a-SM actin and F-actin.
Two of the tested dressings induced cytotoxic effects. They were found to inhibit cell growth (greater than 60%) and to disturb cell shape and cytoskeletal differentiation. Urgotul and the remaining three dressings showed no effect on proliferation. However, some of them modified fibroblast morphology and affected F-actin distribution.
Depending on their nature and components, wound dressings may respect or affect fibroblast behaviour in vitro (proliferation, morphology and a-SM actin and F-actin distribution). The significance of these in vitro observed findings require further investigations.
我们比较了一种新型非粘性伤口敷料优格托（Urgotul）与其他五种伤口敷料对培养的人成纤维细胞的影响。优格托是一种水胶体敷料；对照敷料包括浸渍纱布和现代伤口敷料。
使用单层培养物研究成纤维细胞的形态和生长。使用培养的真皮替代物的贝尔模型研究肌成纤维细胞分化。这些培养物用α-平滑肌肌动蛋白（α-SM actin）和丝状肌动蛋白（F-actin）进行标记。
两种受试敷料诱导了细胞毒性作用。发现它们抑制细胞生长（超过60%）并扰乱细胞形态和细胞骨架分化。优格托和其余三种敷料对增殖没有影响。然而，其中一些改变了成纤维细胞形态并影响了F-肌动蛋白分布。
根据其性质和成分，伤口敷料可能在体外影响或不影响成纤维细胞行为（增殖、形态以及α-平滑肌肌动蛋白和F-肌动蛋白分布）。这些体外观察结果的意义需要进一步研究。
