Suppr超能文献

编码翻译延伸因子EF-1α的拟南芥A1基因的激活过程在被子植物中是保守的。

The activation process of Arabidopsis thaliana A1 gene encoding the translation elongation factor EF-1 alpha is conserved among angiosperms.

作者信息

Curie C, Liboz T, Montané M H, Rouan D, Axelos M, Lescure B

机构信息

Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, Castanet-Tolosan, France.

出版信息

Plant Mol Biol. 1992 Apr;18(6):1083-9. doi: 10.1007/BF00047711.

Abstract

In Arabidopsis thaliana, the activation process of the A1 EF-1 alpha gene depends on several elements. Using the GUS reporter gene, transient expression experiments have shown that mutations of upstream cis-acting elements of the A1 promoter, or the deletion of an intron located within the 5' non-coding region, similarly affect expression in dicot or monocot protoplasts. The results reported here strongly suggest that this 5' intron is properly spliced in Zea mays. We show that two trans-acting factors, specifically interacting with an upstream activating sequence (the TEF 1 box), are present in nuclear extracts prepared from A. thaliana, Brassica rapa, Nicotiana tabacum and Z. mays. In addition, a DNA sequence homologous to the TEF 1 box, found at approximately the same location within a Lycopersicon esculentum EF-1 alpha promoter, interacts with the same trans-acting factors. Homologies found between the A. thaliana and L. esculentum TEF 1 box sequences have allowed us to define mutations of this upstream element which affect the interaction with the corresponding trans-acting factors. These results support the notion that the activation processes of A. thaliana EF-1 alpha genes have been conserved among angiosperms and provide interesting data on the functional structure of the TEF 1 box.

摘要

在拟南芥中,A1 EF-1α基因的激活过程取决于多个元件。利用GUS报告基因进行的瞬时表达实验表明,A1启动子上游顺式作用元件的突变,或位于5'非编码区的一个内含子的缺失,同样会影响双子叶或单子叶原生质体中的表达。本文报道的结果强烈表明,这个5'内含子在玉米中能够正确剪接。我们发现,从拟南芥、芜菁、烟草和玉米制备的核提取物中存在两种与上游激活序列(TEF 1盒)特异性相互作用的反式作用因子。此外,在番茄EF-1α启动子内大致相同位置发现的与TEF 1盒同源的DNA序列,也与相同的反式作用因子相互作用。拟南芥和番茄TEF 1盒序列之间的同源性使我们能够确定该上游元件的突变,这些突变会影响与相应反式作用因子的相互作用。这些结果支持了拟南芥EF-1α基因的激活过程在被子植物中保守的观点,并为TEF 1盒的功能结构提供了有趣的数据。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验