Barun Süreyya, Ekingen Gülşen, Mert Vural Ismail, Türkyilmaz Zafer, Başaklar Can, Kale Nuri, Sevim Ercan Zeynep, Sarioğlu Yusuf
Department of Pharmacology, Medical School, Gazi University, Beşevler, 06510 Ankara, Turkey.
Naunyn Schmiedebergs Arch Pharmacol. 2005 May;371(5):351-8. doi: 10.1007/s00210-005-1073-3. Epub 2005 Jul 8.
It is not known whether there is an impairment in vas deferens motility after unilateral testicular torsion/detorsion. Therefore, we aimed to determine whether the electrical field stimulation (EFS)-evoked biphasic contractions are altered in ipsilateral and contralateral rat vasa deferentia obtained from animals exposed to the unilateral testicular torsion/detorsion procedure. We also evaluated the effects of melatonin (MLT), which is a strong antioxidant, on these contractile responses. Rats were subjected to torsion of the left testis for 2 h and then detorsion was performed. Contractility studies were carried out 2 h or 24 h after detorsion. Vas deferens strips were prepared from both the ipsilateral and the contralateral site 2 h or 24 h after the detorsion procedure to record EFS-evoked biphasic twitch responses. The same experimental protocol was repeated for the MLT-treated rats. Both phases of EFS-evoked contractions were decreased after torsion/detorsion in the ipsilateral vas deferens. MLT treatment increased torsion/detorsion-induced reduction of both phases of contractions after 2 h and 24 h. In the contralateral vas deferens, the first phase of EFS-evoked contractions was not changed, while the second phase of contractions was diminished 2 h and 24 h after detorsion. Although MLT decreased the second phase of contractions 2 h and 24 h after detorsion, it reduced the first phase of contractions only 2 h after detorsion. These results suggest that MLT produces an inhibition on EFS-evoked biphasic twitch responses in the ipsilateral and contralateral rat vasa deferentia following unilateral testicular torsion/detorsion in the rat.
单侧睾丸扭转/复位后输精管运动功能是否受损尚不清楚。因此，我们旨在确定从接受单侧睾丸扭转/复位手术的动物获得的同侧和对侧大鼠输精管中，电场刺激（EFS）诱发的双相收缩是否发生改变。我们还评估了强抗氧化剂褪黑素（MLT）对这些收缩反应的影响。将大鼠左侧睾丸扭转2小时，然后进行复位。在复位后2小时或24小时进行收缩性研究。在复位手术后2小时或24小时，从同侧和对侧制备输精管条，以记录EFS诱发的双相抽搐反应。对接受MLT治疗的大鼠重复相同的实验方案。同侧输精管扭转/复位后，EFS诱发收缩的两个阶段均降低。MLT治疗在2小时和24小时后增加了扭转/复位引起的两个收缩阶段的降低。在对侧输精管中，EFS诱发收缩的第一阶段没有变化，而复位后2小时和24小时收缩的第二阶段减弱。虽然MLT在复位后2小时和24小时降低了收缩的第二阶段，但它仅在复位后2小时降低了收缩的第一阶段。这些结果表明，在大鼠单侧睾丸扭转/复位后，MLT对同侧和对侧大鼠输精管中EFS诱发的双相抽搐反应产生抑制作用。
