Boldyrev I A, Molotkovskiĭ Iul G
Bioorg Khim. 2005 May-Jun;31(3):331-4. doi: 10.1007/s11171-005-0041-5.
Three new fluorescent probes were synthesized for improving the method of studying donor-donor energy migration (DDEM). Each probe has two identical fluorescent 7-diethylaminocoumarin-3-carbonyl groups attached to a rigid bisteroid dodecacyclic spacer through additional inserts. In two probes, the inserts are beta-Ala and L-Ser residues, which provide for a different nearest environment of the fluorophores. The third probe has identical beta-Ala inserts. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 3; see also http://www.maik.ru.
合成了三种新型荧光探针,以改进供体-供体能量迁移(DDEM)的研究方法。每个探针都有两个相同的荧光7-二乙氨基香豆素-3-羰基基团,通过额外的插入基团连接到一个刚性双甾体十二环间隔基上。在两种探针中,插入基团是β-丙氨酸和L-丝氨酸残基,这为荧光团提供了不同的最邻近环境。第三种探针具有相同的β-丙氨酸插入基团。论文英文版:《俄罗斯生物有机化学杂志》,2005年,第31卷,第3期;另见http://www.maik.ru 。