Monnet Fabien, Bordas François, Deluchat Véronique, Chatenet Philippe, Botineau Michel, Baudu Michel
Laboratoire des sciences de l'eau et de l'environnement, Faculté des sciences, Université de Limoges, 123 Avenue Albert Thomas, F-87060 Limoges, France.
Environ Pollut. 2005 Dec;138(3):455-61. doi: 10.1016/j.envpol.2005.04.019.
Laboratory experiments were conducted to investigate the potential use of the aquatic lichen Dermatocarpon luridum as bioindicator of copper pollution. Lichen thalli were exposed to 0.00, 0.25, 0.50, 0.75 and 1.00 mM copper in synthetic freshwater to solve the problems of metal bioavailability. The mineral composition of this media was prepared so that it corresponded to the ion composition of natural waters in D. luridum ecosystems. Sequential elution procedures using NiCl2 or Na2-EDTA (20 mM) were used to determine the distribution of metals at different cellular sites. The copper concentration extracted from thalli was correlated with pollution intensity, the greater correlation being with the Na2-EDTA extractant. The malondialdehyde concentration in thalli can be used as indicator of copper pollution; however, similar membrane degradation was observed for 0.25 and 0.50 mM copper and for 0.75 and 1.00 mM copper.
开展了实验室实验，以研究水生地衣暗黑皮果衣作为铜污染生物指示物的潜在用途。为解决金属生物可利用性问题，将地衣叶状体暴露于合成淡水中0.00、0.25、0.50、0.75和1.00 mM的铜环境中。该培养基的矿物质成分是按照其对应于暗黑皮果衣生态系统中天然水体的离子组成来配制的。使用NiCl2或Na2-EDTA（20 mM）的连续洗脱程序来确定不同细胞部位的金属分布。从叶状体中提取的铜浓度与污染强度相关，与Na2-EDTA萃取剂的相关性更强。叶状体中的丙二醛浓度可用作铜污染的指标；然而，在0.25和0.50 mM铜以及0.75和1.00 mM铜处理组中观察到了类似的膜降解情况。
