Nissinen M J, Castrèn E, Holthöfer H, Panula P
Department of Anatomy, University of Helsinki, Finland.
Cell Tissue Res. 1992 Feb;267(2):233-9. doi: 10.1007/BF00302960.
An antiserum against conjugated histamine and two oligonucleotide probes that detect the mRNA encoding L-histidine decarboxylase (HDC) involved in histamine synthesis were used to study the appearance of histamine and its location in the kidneys of fetal, newborn and young postnatal rats and in the kidneys of pregnant rats. On embryonic days 16 and 18 (E16 and E18), some HA-immunoreactive (HA-ir) cells were found within the largest S-shaped bodies. Histamine was found to appear rapidly between the 18th and 20th embryonic days in the convoluted tubules of the kidneys. On postnatal day 0 (P0), the distal convoluted tubules and collecting ducts exhibited bright fluorescence, the intensity of which decreased quickly so that it was faint on day P4 and absent at later stages. In kidneys of pregnant rats HA-ir was found in the epithelium of both the Bowman's capsule, collecting ducts and in a few cells within the tubules. Nonuniform HA-ir was also detected within glomeruli. No evidence for the presence of L-histidine decarboxylase mRNA in kidneys of fetuses or pregnant rats was seen. It is concluded that distinct structures in the developing rat kidney contain histamine during a period around birth from day E20 to day P4. In the pregnant rat, the epithelium that is in direct contact with the urine flow is immunoreactive for histamine from day 16 to 20 of pregnancy. The results suggest that histamine is not synthesized locally in the kidneys but rather originates from other tissues.
使用一种针对共轭组胺的抗血清和两种寡核苷酸探针来研究组胺的出现及其在胎鼠、新生鼠和幼龄出生后大鼠肾脏以及孕鼠肾脏中的位置。这两种寡核苷酸探针可检测参与组胺合成的L-组氨酸脱羧酶（HDC）的mRNA。在胚胎第16天和18天（E16和E18），在最大的S形体内发现了一些组胺免疫反应性（HA-ir）细胞。发现组胺在胚胎第18天至20天期间在肾脏的曲小管中迅速出现。在出生后第0天（P0），远曲小管和集合管呈现明亮荧光，其强度迅速降低，以至于在P4天时变得微弱，在后期消失。在孕鼠的肾脏中，在鲍曼囊、集合管的上皮以及小管内的一些细胞中发现了HA-ir。在肾小球内也检测到不均匀的HA-ir。在胎儿或孕鼠的肾脏中未发现L-组氨酸脱羧酶mRNA存在的证据。得出的结论是，在出生前后从E20天到P4天这段时间内，发育中的大鼠肾脏中的不同结构含有组胺。在孕鼠中，从妊娠第16天到20天，与尿液流动直接接触的上皮对组胺具有免疫反应性。结果表明，组胺不是在肾脏中局部合成的，而是起源于其他组织。
