Nissinen M J, Panula P
Department of Anatomy, University of Helsinki, Finland.
Agents Actions. 1991 May;33(1-2):177-80. doi: 10.1007/BF01993160.
An antiserum against conjugated histamine was used to reveal the location and time of the appearance of the amine in different organs during the development of fetal and early postnatal rats. Tissues were fixed in 4% 1-ethyl-3(3-dimethyl-aminopropyl) carbodiimide and standard indirect immunofluorescence technique was used. Histamine-immunoreactivity (HA-ir) was first detected in the rat fetus on the 10th embryonic day (day E10) in the embryonic liver. The liver remained immunoreactive during the second half of the fetal life. In addition to some small HA-ir cells in the stomach wall and in the kidneys on day E16, the papillary muscles of the fetal heart also exhibited patchy immunofluorescence at this stage. On day E18, HA-ir cells appeared in many other organs including lungs, gastric mucosa and smooth muscle cells of the stomach wall, heart muscle and subcutaneous tissue. The distribution of HA-ir in fetal tissues was most extensive on day E20 when the kidney tubules and the skin also exhibited bright fluorescence. These results suggest that histamine is widely distributed during fetal development in different cell types.
一种针对结合组胺的抗血清被用于揭示在胎鼠和出生后早期大鼠发育过程中，胺类物质在不同器官中的出现位置和时间。组织用4% 1-乙基-3（3-二甲基氨基丙基）碳二亚胺固定，并采用标准间接免疫荧光技术。组胺免疫反应性（HA-ir）首先在胚胎第10天（E10）的胚胎肝脏中被检测到。在胎儿期的后半段，肝脏一直保持免疫反应性。在E16时，除了胃壁和肾脏中有一些小的HA-ir细胞外，胎儿心脏的乳头肌在这个阶段也表现出斑片状免疫荧光。在E18时，HA-ir细胞出现在许多其他器官中，包括肺、胃黏膜、胃壁平滑肌细胞、心肌和皮下组织。在E20时，胎儿组织中HA-ir的分布最为广泛，此时肾小管和皮肤也呈现明亮的荧光。这些结果表明，组胺在胎儿发育过程中广泛分布于不同的细胞类型中。
