Wang J W, Wu J H, Huang W Y, Tan R X
Institute of Functional Biomolecules, School of Life Sciences, Nanjing University, Nanjing 210093, China.
Bioresour Technol. 2006 Mar;97(5):786-9. doi: 10.1016/j.biortech.2005.03.025. Epub 2005 Jul 11.
The effects of the carbon and nitrogen sources, initial pH and incubation temperature on laccase production by the endophytic fungus Monotospora sp. were evaluated. The optimal temperature and initial pH for laccase production by Monotospora sp. in submerged culture were found to be 30 degrees C and 8.5, respectively. Maltose (2 g l(-1)) and ammonium tartrate (10 g l(-1)) were the most suitable carbon and nitrogen source for laccase production. Under optimal culture medium, the maximum laccase activity was determined to be 13.55 U ml(-1), which was approximately four times higher than that in basal medium. This is the first report on laccase production by an endophytic fungus.
评估了碳源、氮源、初始pH值和培养温度对内生真菌单孢霉属（Monotospora sp.）漆酶产生的影响。发现单孢霉属在液体培养中产生漆酶的最适温度和初始pH值分别为30℃和8.5。麦芽糖（2 g l(-1)）和酒石酸铵（10 g l(-1)）是最适合漆酶产生的碳源和氮源。在最佳培养基条件下，测定的最大漆酶活性为13.55 U ml(-1)，约为基础培养基中漆酶活性的四倍。这是关于内生真菌产生漆酶的首次报道。
