Department of Environmental Science, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Lucknow 226025, Uttar Pradesh, India.
Appl Biochem Biotechnol. 2012 Feb;166(4):1082-94. doi: 10.1007/s12010-011-9496-4. Epub 2011 Dec 28.
A novel laccase producing Basidiomycete Peniophora sp. (NFCCI-2131) was isolated from pulp and paper mill effluent. The optimal temperature and initial pH for laccase production by the isolate in submerged culture were found to be 30 and 4.6° C, respectively. Maltose (20 g l⁻¹) and tryptone (1.0 g l⁻¹) were the most suitable carbon and nitrogen sources for laccase production. Cu²⁺ (1.0 mM) and veratryl alcohol induced maximum laccase production giving 6.6 and 6.07 U/ml laccase activity, respectively. Under optimised culture conditions, 7.6 U/ml activity was obtained, which was 2.4 times higher than that was achieved in basal medium. An evaluation of the delignification efficiency of the crude enzyme in the presence of redox mediators [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and (1-hydroxybenzotriazole)] revealed structural changes in lignin and existence of many active centres for both chemical and biological degradation of lignin following enzymatic treatment.
从纸浆和造纸厂废水中分离到一株产漆酶的担子菌 Peniophora sp.（NFCCI-2131）。该分离株在液体培养中产漆酶的最适温度和初始 pH 值分别为 30°C 和 4.6°C。麦芽糖（20 g/L）和胰蛋白胨（1.0 g/L）是产漆酶的最佳碳源和氮源。Cu²⁺（1.0 mM）和藜芦醇诱导产生最大漆酶活性，分别为 6.6 和 6.07 U/ml 漆酶活性。在优化的培养条件下，获得了 7.6 U/ml 的酶活，比基础培养基中的酶活提高了 2.4 倍。在存在氧化还原介体[2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）和（1-羟基苯并三唑）]的情况下，对粗酶的木质素脱除效率进行评估，结果表明木质素结构发生了变化，并且存在许多活性中心，可进行木质素的化学和生物降解。
