Xu Zhicheng, Bai Guan, Dong Chuan
Department of Chemistry, Shanxi University, Taiyuan 030006, PR China.
Bioorg Med Chem. 2005 Oct 15;13(20):5694-9. doi: 10.1016/j.bmc.2005.06.023.
The interaction of a new intramolecular charge transfer probe, namely 4'-dimethylamino-2,5-dihydroxychalcone (DMADHC), with calf thymus DNA has been studied. Compared to the spectral characteristics of the free form in aqueous solution, the fluorescence of DMADHC enhanced dramatically accompanying a blueshift of the emission maxima in the presence of DNA. The absorption and fluorescence spectra, salt concentration effect, KI quenching, fluorescence polarization, and DNA denaturation experiments were given. These results give evidence that the DMADHC molecule is inserted into the base-stacking domain of the DNA double helix. The intrinsic binding constant and the binding site number were estimated. The analytical characteristics were also given.
研究了一种新型分子内电荷转移探针，即4'-二甲基氨基-2,5-二羟基查耳酮（DMADHC）与小牛胸腺DNA的相互作用。与水溶液中游离形式的光谱特征相比，在DNA存在下，DMADHC的荧光显著增强，同时发射最大值发生蓝移。给出了吸收光谱、荧光光谱、盐浓度效应、KI猝灭、荧光偏振和DNA变性实验。这些结果表明DMADHC分子插入到DNA双螺旋的碱基堆积区域。估算了固有结合常数和结合位点数。还给出了分析特性。
