Bômont L, Bilger A, Boyet S, Vert P, Nehlig A
Pathologie et Biologie du Développement Humain, INSERM U.273 Universitè de Nancy I, France.
Brain Res Dev Brain Res. 1992 Mar 20;66(1):33-45. doi: 10.1016/0165-3806(92)90137-l.
The quantitative autoradiographic 2-[14C]-deoxyglucose technique (2-DG) was applied to measure the effects of an acute hypoxic exposure on local cerebral metabolic rates for glucose (LCMRglcs) in the 10 (P10)-, 14 (P14)-, and 21 (P21)-day-old rat. The animals were exposed to hypoxic (7% O2/93% N2) or control gas mixture (21% O2/79% N2) for 20 min before the initiation and for the duration of the 2-DG procedure. Lumped constants were not affected by hypoxia at any age. At P10, the exposure to the hypoxic gas mixture induced a generalized increase in LCMRglc which affected 41 structures of the 45 studied. At P14, average cerebral glucose utilization was similar in hypoxic and control rats. LCMRglc increased in 5 areas and decreased in 11 regions, mainly brainstem and respiratory areas in hypoxic rats. Finally, at P21, LCMRglc decreased in 11 structures of hypoxic rats. The increase in LCMRglc in the hypoxic 10-day-old rat likely reflects stimulation of anaerobic glycolysis. Conversely, at P14 and P21, when the brain has become more dependent upon oxygen supply for its energy metabolism, levels of LCMRglc are similar in both groups of animals or decreased in a few structures of hypoxic compared to normoxic rats. The results of the present study show that the immature brain responds to an acute hypoxic insult in a specific way according to its maturational state. They are also in good accordance with the higher resistance of the immature animal to oxygen deprivation.
采用定量放射自显影2-[¹⁴C] -脱氧葡萄糖技术（2-DG），测定急性低氧暴露对10日龄（P10）、14日龄（P14）和21日龄（P21）大鼠局部脑葡萄糖代谢率（LCMRglcs）的影响。在开始2-DG程序之前及程序进行期间，将动物暴露于低氧（7% O₂/93% N₂）或对照气体混合物（21% O₂/79% N₂）中20分钟。在任何年龄，集总常数均不受低氧影响。在P10时，暴露于低氧气体混合物导致LCMRglc普遍升高，在所研究的45个结构中有41个受到影响。在P14时，低氧大鼠和对照大鼠的平均脑葡萄糖利用率相似。低氧大鼠的LCMRglc在5个区域升高，在11个区域降低，主要是脑干和呼吸区域。最后，在P21时，低氧大鼠的11个结构中的LCMRglc降低。低氧10日龄大鼠LCMRglc的升高可能反映了无氧糖酵解的刺激。相反，在P14和P21时，当大脑在能量代谢上变得更依赖于氧气供应时，两组动物的LCMRglc水平相似，或者与常氧大鼠相比，低氧大鼠的一些结构中LCMRglc降低。本研究结果表明，未成熟大脑根据其成熟状态以特定方式对急性低氧损伤做出反应。它们也与未成熟动物对缺氧的更高抵抗力高度一致。
