Munjal S K, Tripathi B N, Paliwal O P
IVD GmbH, School of Veterinary Medicine, Bischofsholer Damm 15, Hannover, 30173, Germany.
Vet Pathol. 2005 Jul;42(4):427-36. doi: 10.1354/vp.42-4-427.
A dose of 10(10) Mycobacterium avium subspecies paratuberculosis was administered orally on seven occasions to produce experimental paratuberculosis infection in 10 5-8-week-old goat kids. Bacteriological, immunological, and histopathological changes, their relationships, and the efficacy of the commonly used diagnostic methods were studied during the progressive disease up to 270 days postinfection (DPI). Significant lymphocyte proliferative responses in the peripheral blood of five goats were detected as early as 60 DPI. A lymphoproliferative test was also performed on lymphocytes purified from different compartments of the guts of five infected and five control goats. Significant proliferative responses were observed in lymphocytes of jejunal compartments of all five goats, of which four had also significant lymphocyte proliferation in the blood. The ileal lymphocytes from two goats, one each at 120 and 270 DPI, had significant proliferation. The histological lesions were mainly observed in the gut-associated lymphoid tissues of the ileocecal valve, the ileum, and the terminal jejunum. Acid-fast bacilli were demonstrated in the lesions of two goats at 60 and 210 DPI. Bacterial culture showed poor sensitivity, detecting positive results for only one goat in the fecal and tissue samples at 210 DPI, whereas polymerase chain reaction (PCR) detected one goat in fecal sample at 210 DPI and two goats in tissue samples at 60 and 210 DPIs, respectively. Enzyme-linked immunosorbent assay and agar gel immunodiffusion test were found to be 100% sensitive from 180 and 210 DPI onwards, respectively.
将10(10)剂量的副结核分枝杆菌口服给药7次，以在10只5 - 8周龄的山羊羔中引发实验性副结核感染。在感染后长达270天的疾病进展过程中，研究了细菌学、免疫学和组织病理学变化、它们之间的关系以及常用诊断方法的有效性。早在感染后60天，就在5只山羊的外周血中检测到显著的淋巴细胞增殖反应。还对从5只感染山羊和5只对照山羊肠道不同部位纯化的淋巴细胞进行了淋巴细胞增殖试验。在所有5只山羊的空肠部位的淋巴细胞中观察到显著的增殖反应，其中4只山羊的血液中也有显著的淋巴细胞增殖。2只山羊的回肠淋巴细胞在感染后120天和270天各有1只出现显著增殖。组织学病变主要见于回盲瓣、回肠和空肠末端的肠道相关淋巴组织。在感染后60天和210天，在2只山羊的病变中发现了抗酸杆菌。细菌培养显示敏感性较差，在感染后210天仅在1只山羊的粪便和组织样本中检测到阳性结果，而聚合酶链反应（PCR）分别在感染后210天的粪便样本中检测到1只山羊，在感染后60天和210天的组织样本中检测到2只山羊。酶联免疫吸附试验和琼脂凝胶免疫扩散试验分别从感染后180天和210天起显示100%的敏感性。
