Burnside D M, Rowley B O
Quakertown Veterinary Clinic, PA 18951.
Am J Vet Res. 1994 Apr;55(4):465-6.
A commercial rapid-absorbed ELISA developed to detect antibodies to Mycobacterium paratuberculosis in bovine serum was modified for use with goat serum. Diagnostic sensitivity was evaluated, using a group of 163 goats from a herd with endemic paratuberculosis. Blood and fecal samples were obtained simultaneously, and prevalence of shedding of M paratuberculosis in the feces was estimated by detection of DNA of the mycobacterial insertion sequence, IS900, using a commercial test kit. Diagnostic specificity was evaluated, using blood samples from a total of 123 goats in 10 herds that were considered clinically free of paratuberculosis. The IS900 DNA was detected in 35 of the 163 goats (21%) from the infected herd. Serum antibody to M paratuberculosis was detected in 19 of the 35 IS900 DNA-positive goats, for apparent sensitivity of 54%. Serum antibody was detected in 18 of the 128 IS900 DNA-negative goats from the infected herd. Negative results for serum antibody to M paratuberculosis were obtained for all 123 goats from the herds that were considered clinically free of paratuberculosis.
一种用于检测牛血清中副结核分枝杆菌抗体的商业化快速吸收酶联免疫吸附测定法（ELISA）经过改良后用于山羊血清检测。使用来自一个地方流行副结核的羊群中的163只山羊评估诊断敏感性。同时采集血液和粪便样本，并使用商用检测试剂盒通过检测分枝杆菌插入序列IS900的DNA来估计粪便中副结核分枝杆菌的排泄率。使用来自10个被认为临床上无副结核的羊群中的总共123只山羊的血液样本评估诊断特异性。在来自感染羊群的163只山羊中有35只（21%）检测到IS900 DNA。在35只IS900 DNA阳性山羊中有19只检测到副结核分枝杆菌血清抗体，表观敏感性为54%。在来自感染羊群的128只IS900 DNA阴性山羊中有十八只检测到血清抗体。对于所有来自被认为临床上无副结核的羊群的123只山羊，副结核分枝杆菌血清抗体检测结果均为阴性。
