Dawson D W, Fish D I, Shackleton P
Haematology Department, North Manchester General Hospital.
Clin Lab Haematol. 1992;14(1):47-52.
The accuracy of methods used to assay serum ferritin was determined in two ways. In one a serum, to which the UK ferritin standard had been added, was issued to the participants in an inter-regional quality assurance scheme. The overall recovery was close to that expected. For the second assessment a series of sera from individuals of known iron status were issued. Differences between assay results related to the method of assay though the immunoradiometric and ELISA methods gave results which were close to each other. The variation in clinical interpretation applied to assay results when ferritin concentrations around the upper and lower limits of normal were assayed was more than could be accounted for by method imprecision and indicates that inappropriate reference ranges are in common use. 'Indeterminate', a proper clinical interpretation when the imprecision of a technique should prevent a definite response, was inadequately used.
用于测定血清铁蛋白的方法的准确性通过两种方式来确定。其一，将添加了英国铁蛋白标准品的一份血清发放给参与区域间质量保证计划的人员。总体回收率接近预期。对于第二项评估，发放了一系列来自已知铁状态个体的血清。尽管免疫放射分析和酶联免疫吸附测定方法得出的结果相近，但测定结果之间的差异与测定方法有关。当测定铁蛋白浓度处于正常上限和下限附近时，应用于测定结果的临床解释差异大于方法不精密度所能解释的范围，这表明常用的参考范围不合适。“不确定”，即当一项技术的不精密度应阻止给出明确反应时的一种恰当临床解释，使用不充分。
